09-070-25UG | Anti-ATR (rabbit polyclonal)

09-070-25UG
25 μg  
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      Description
      Catalogue Number09-070-25UG
      DescriptionAnti-ATR (rabbit polyclonal)
      Alternate Names
      • Ataxia telangiectasia and Rad3-related protein
      • FRAP-related protein 1
      • FRAP-related protein-1
      • MEC1
      • mitosis entry checkpoint 1
      Background InformationATR (Ataxia Telangiectasia and Rad3-related kinase) is a protein kinase that regulates cell cycle checkpoints and DNA repair. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1). ATR belongs to the PI3/PI4-kinase family, and is most closely related to ATM (Ataxia Telangiectasia Mutated kinase).
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit serum in 0.1M Tris-Glycine (pH7.4) and 150mM NaCl containing 0.05% sodium azide.
      Applications
      ApplicationAnti-ATR Antibody detects level of ATR & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: A 1:500 dilution from a previous lot of this antibody was used to was detect ATR in HeLa, NIH3T3, and C6 cell lysates.
      Biological Information
      ImmunogenKLH linear peptide corresponding to amino acids 1887-1902 of ATR.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes ATR.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      • Horse
      • Bovine
      Species Reactivity NoteProven to react with human, mouse, and rat. Predicted to react with dog, horse, and cow based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
      Gene Symbol
      • EC 2.7.11.1
      • FRP1
      • MEC1
      • SCKL
      • SCKL1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      COFACTOR: Manganese.
      ENZYME REGULATION: Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine, wortmannin and LY294002.
      SUBUNIT STRUCTURE: Forms an heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164.
      SUBCELLULAR LOCATION: Nucleus. Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
      PTM: Phosphorylated; autophosphorylates in vitro.
      INVOLVEMENT IN DISEASE: Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
      SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      Molecular Weight250 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HeLa cell lysate.
      Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect ATR in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

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