Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition. Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.
A431 cells Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Protein G purified mouse IgGs in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20°C.
Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) is a Mouse Monoclonal Antibody for detection of Cyclin B1 also known as G2/mitotic-specific cyclin B1 & has been validated in IP & WB.
Immunoprecipitation: 2 μg of a previous lot immunoprecipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate as determined by a subsequent immunoblot of the immunoprecipitate using anti-cdk1/cdc2 (PSTAIR), (Catalog # 06-923).
Recombinant human cyclin B1
Please refer to lot specific datasheet.
This antibody is specific for human cyclin B1, Mr 58 kDa. Does not cross-react with cyclin A or cyclin D.
Species Reactivity Note
In addition to human, weak species cross-reactivity was observed with mouse.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
FUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition. SIZE: 433 amino acids; 48337 Da SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome. DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis. PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
Safety Information according to GHS
Product Usage Statements
Routinely evaluated by western blot analysis on RIPA lysate from human A431 carcinoma cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected cyclin B1 in RIPA lysates from human A431 carcinoma cells.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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