MABS1805 | Anti-Dach1 Antibody, clone 1F6.1

MABS1805
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS1805
      DescriptionAnti-Dach1 Antibody, clone 1F6.1
      Alternate Names
      • Dachshund homolog 1
      Background InformationDachshund homolog 1 (UniProt: Q9UI36; also known as Dach1) is encoded by the DACH1 (also known as DACH) gene (Gene ID: 1602) in human. Dach1 is a transcription factor that is involved in regulation of organogenesis. Four isoforms of Dach1 have been reported that are produced by alternative splicing. It is shown to be widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart and isoform 4 is found in spleen. Clone 1F6.1 exhibits reactivity with all four isoforms. Dach1 is reported to act as regulator of SIX1, SIX6, and probably SIX5. It also acts as a a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Dach1 is shown to Inhibit TGF-beta signaling through interaction with SMAD4 and NCOR1. It binds to chromatin DNA via its DACHbox-N domain, which forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain. PELP1 (proline, glutamic acid, and leucine rich protein 1) serves as a Dach1-binding protein and the carboxyl terminus of Dach1 is required for binding to PELP1. Dach1 is reported to prevent the proliferation of cancerous cells due to being a natural repressor of estrogen receptors (ER) in the normal function of mammary tissue. It inhibits ER alpha transcriptional activity. (Ref.: Popov, VM et al. (2009). Cancer Res. 69(14); 5752-60). +D1
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Dach1, clone 1F6.1, Cat. No. MABS1805, is a mouse monoclonal antibody that detects Dach1 in human and murine cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dach1 in human bone marrow and human colon cancer tissues.

      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Dach1 in Neuro-2a cell lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment coreesponding to 60 amino acids from the C-termnal region of human Dach1.
      Clone1F6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1F6.1 detects all isoforms of Dach1 in human and murine cells. It targets an epitope within 60 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DACH1
      • DACH
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~78 kDa observed; 78.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SHSY-5Y cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Dach1 in 10 µg of SHSY-5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications