MABE1067-25UG Anti-ESET (SETDB1) Antibody, clone F0808

新着MABE1067-25UG
25 μg  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABE1067-25UG
      DescriptionAnti-ESET (SETDB1) Antibody, clone F0808
      Alternate Names
      • Histone-lysine N-methyltransferase SETDB1
      • EC: 2.1.1.43
      • ERG-associated protein with SET domain
      • SET domain bifurcated 1
      Background InformationHistone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1, SETDB1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in murine species. SETDB1 is a ubiquitously expressed histone methyltransferase with multiple zinc-binding sites that specifically trimethylates lysine 9 of histone H3 immediately downstream of transcription start sites marked with H3K4me3 to establish the bivalent domain. It is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by Krüppel-associated box (KRAB) zinc-finger proteins. The H3K4/H3K9me3 signature keeps RNA polymerase II (Pol II) paused at the proximal promoter and maintains gene expression at low levels, but in a poised state that is ready for activation in lineage-committed cells. It functions mainly in euchromatin regions, thereby playing a key role in the silencing of euchromatic genes. It probably acts by forming a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone lysine 9 trimethylation. SETDB1 is reported to inhibit adipogenesis and its depletion induces preadipocyte differentiation and adipogenesis in mesenchymal stem cells and preadipocytes. Expression of Cebpa and Pparg genes that are its key targets of SETDB1 is enhanced when SETDB1 is knocked down in undifferentiated cells. Murine SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and pre-SET (aa 744-817), SET (aa 820-1282), and post-SET (aa 1291-1307) domains that are required for its methyltransferase activity. (Ref.: Matsumura, Y., et al. (2015). Mol. Cell 60(4); 584-596).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
      Applications
      ApplicationAnti-ESET (SETDB1), clone F0808, Cat. No. MABE1067, is a highly specific mouse monoclonal antibody that targets Histone-lysine N-methyltransferase SETDB1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunoprecipitation
      • Western Blotting
      Application NotesChromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
      CloneF0808
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Setdb1
      • Eset
      • Kiaa0067
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~180/200 kDa observed; 144.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 129/S6/SvEv mouse embryonic stem cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected ESET (SETDB1) in 129/S6/SvEv mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications

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