07-371 Anti-Histone H2B Antibody

07-371
200 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-371
      ReplacesMABE15
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2B Antibody
      Alternate Names
      • H2B
      • Histone H2B
      • H2B histone family, member Q
      • histone 2
      • H2be
      • histone cluster 2
      • H2be
      Background InformationHistone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
      References
      Product Information
      FormatPurified
      Control
      • Acid extract from either sodium butyrate or colcemid treated HeLa cells.
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      Species Reactivity NoteHuman, Chicken. Predicted to react with Yeast, Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
      Gene Symbol
      • GL105
      • H2B
      • H2B-GL105
      • H2B.1
      • H2B.q
      • H2B/q
      • H2BFQ
      • MGC119802
      • MGC119804
      • MGC129733
      • MGC129734
      • OTTHUMP00000013920
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 126 amino acids; 13908 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
      SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
      Molecular Weight~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Chicken Core Histone.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Histone H2B Antibody (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-Histone H2B Antibody 試験成績書(CoA)

      タイトルロット番号
      Anti-Histone H2B - 24469082446908
      Anti-Histone H2B - 19626531962653
      Anti-Histone H2B - 19939241993924
      Anti-Histone H2B - 20731352073135
      Anti-Histone H2B - 21872232187223
      Anti-Histone H2B - 2244222442
      Anti-Histone H2B - 22956592295659
      Anti-Histone H2B - 2751427514
      Anti-Histone H2B - 3021630216
      Anti-Histone H2B - 31984563198456

      参考資料

      参考資料の概要アプリケーション動物種Pub Med ID
      The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth.
      Ohashi, M; Holthaus, AM; Calderwood, MA; Lai, CY; Krastins, B; Sarracino, D; Johannsen, E
      PLoS pathogens  11  e1004822  2015

      概要を表示する
      25855980 25855980
      The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.
      Creamer, KM; Job, G; Shanker, S; Neale, GA; Lin, YC; Bartholomew, B; Partridge, JF
      Molecular and cellular biology  34  2046-61  2014

      概要を表示する
      Western Blotting24662054 24662054
      TAp73 is essential for germ cell adhesion and maturation in testis.
      Holembowski, L; Kramer, D; Riedel, D; Sordella, R; Nemajerova, A; Dobbelstein, M; Moll, UM
      The Journal of cell biology  204  1173-90  2014

      概要を表示する
      24662569 24662569
      Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory.
      Hait, NC; Wise, LE; Allegood, JC; O'Brien, M; Avni, D; Reeves, TM; Knapp, PE; Lu, J; Luo, C; Miles, MF; Milstien, S; Lichtman, AH; Spiegel, S
      Nature neuroscience  17  971-80  2014

      概要を表示する
      Western BlottingMouse24859201 24859201
      Cotranscriptional histone H2B monoubiquitylation is tightly coupled with RNA polymerase II elongation rate.
      Fuchs, G; Hollander, D; Voichek, Y; Ast, G; Oren, M
      Genome research  24  1572-83  2014

      概要を表示する
      25049226 25049226
      A human artificial chromosome recapitulates the metabolism of native telomeres in mammalian cells.
      Wakai, M; Abe, S; Kazuki, Y; Oshimura, M; Ishikawa, F
      PloS one  9  e88530  2014

      概要を表示する
      Western Blotting24558398 24558398
      Nucleolar integrity is required for the maintenance of long-term synaptic plasticity.
      Allen, KD; Gourov, AV; Harte, C; Gao, P; Lee, C; Sylvain, D; Splett, JM; Oxberry, WC; van de Nes, PS; Troy-Regier, MJ; Wolk, J; Alarcon, JM; Hernández, AI
      PloS one  9  e104364  2014

      概要を表示する
      Mouse25089620 25089620
      dRYBP counteracts chromatin-dependent activation and repression of transcription.
      Fereres, S; Simón, R; Mohd-Sarip, A; Verrijzer, CP; Busturia, A
      PloS one  9  e113255  2014

      概要を表示する
      Western Blotting25415640 25415640
      The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast.
      Mbogning, J; Nagy, S; Pagé, V; Schwer, B; Shuman, S; Fisher, RP; Tanny, JC
      PLoS genetics  9  e1004029  2013

      概要を表示する
      24385927 24385927
      Human CAF-1-dependent nucleosome assembly in a defined system.
      Kadyrova, LY; Rodriges Blanko, E; Kadyrov, FA
      Cell cycle (Georgetown, Tex.)  12  3286-97  2013

      概要を表示する
      24036545 24036545

      技術情報

      タイトル
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Life Science Research > Antibodies and Assays > Primary Antibodies