MABC1103 Anti-IDH1/2 Mutant (R132/172) Antibody, clone MsMab-1

MABC1103
100 μg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC1103
      DescriptionAnti-IDH1/2 Mutant (R132/172) Antibody, clone MsMab-1
      Alternate Names
      • Isocitrate dehydrogenase [NADP] cytoplasmic
      • IDH
      • Cytosolic NADP-isocitrate dehydrogenase
      • IDP
      • NADP(+)-specific ICDH
      • Oxalosuccinate decarboxylase
      • Isocitrate dehydrogenase [NADP], mitochondrial
      • ICD-M
      Background InformationIsocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to produce alpha-ketoglutarate and carbon dioxide. There exist three forms of IDHs in human (IDH1-3), with IDH3 utilizing NAD+ as a cofactor and functioning within the citric acid cycle, while IDH1 (EC 1.1.1.42; UniProt P48735) and IDH2 (EC 1.1.1.42; UniProt P48735) utilizing NADP+ as a cofactor and functioning outside the context of the citric acid cycle. Mutated IDH1 and IDH2 convert alpha-ketoglutarate to oncometabolite R(-)-2-hydroxyglutarate (2-HG) in cytosol and mitochondria, respectively. Isocitrate dehydrogenase 1 ⁄ 2 mutations have been reported in gliomas, acute myeloid leukemias (AMLs), cartilaginous tumors, osteosarcoma, Giant cell tumors of bone (GCTB), Ollier disease, and Maffucci syndrome.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-IDH1/2 Mutant (R132/172) Antibody, clone MsMab-1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of IDH1/2 Mutant (R132/172).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IDH1/2 Mutant (R132/172) in human colon, human colorectal cancer, and human ovarian cancer tissue.
      Western Blotting Analysis: The mutant selectivity of a representative lot was tested against purified IDH1/2 MBP fusion constructs as well as lysates from CHO cells expressing exogenously expressed IDH1/2 proteins (Kato Kaneko, M., et al. (2013). Tohoku J Exp Med. 230(2):103-109; Liu, X., et al. (2013). Cancer Med.;2(6):803-814).
      Western Blotting Analysis: A representative lot detected exogenously expressed IDH2- R172S, but not wild-type IDH2 or IDH2-H175Y PA fusion proteins in lysates from transfected U2OS osteosarcoma cells (Kato Kaneko, M., et al. (2014). Cancer Sci.;105(6):744-748).
      Immunohistochemistry Analysis: A representative lot detected IDH2 R172S mutant, but not wild-type IDH1/2, in paraffin-embedded human GCTB (giant cell tumor of bone) tissue sections (Kato Kaneko, M., et al. (2014). Cancer Sci.;105(6):744-748).
      Immunohistochemistry Analysis: A representative lot detected IDH2 R172S mutant, but not wild-type IDH1/2, in paraffin-embedded human osteosarcoma tissue sections (Liu, X., et al. (2013). Cancer Med.;2(6):803-814).
      Biological Information
      ImmunogenKLH-conjugated peptide derived from human IDH1 sequence with R132G mutation.

      EpitopeIDH1-R132/IDH2-R172 mutation site
      CloneMsMAb-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone MsMab-1 recognizes IDH1 with Arg132 mutations (R132AD/E/G/H/M/N/Q/SY) and IDH2 with Arg172 mutations (R172A/C/D/E/G/L/Q/S/Y), but not wild type IDH1/2 or other IDH1/2 mutations (IDH1-R132C/F/I/K/L/P/R/T/V/W; IDH2-H175Y, R172F/H/I/K/M/N/P/T/V/W) by Western blotting and peptide-based ELISA analyses.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IDH1
      • PICD
      • IDH2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~47 to 50 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in IDH2-R172S-expressing U2OS cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected exogenously expressed IDH2-R172S mutant, but not wild-type IDH2, in 10 µg of U2OS cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IDH1/2 Mutant (R132/172) Antibody, clone MsMab-1 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-IDH1/2 Mutant (R132/172) Antibody, clone MsMab-1 試験成績書(CoA)

      タイトルロット番号
      Anti-IDH1/2 Mutant (R132/172), -Q2595853Q2595853
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 - 28955552895555
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 - 31698803169880
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 -28522912852291
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 Monoclonal Antibody3098858
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 Monoclonal Antibody3043533
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 Monoclonal Antibody2965103
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 Monoclonal Antibody3009981
      Anti-IDH1/2 Mutant (R132/172), clone MsMab-1 Monoclonal Antibody2923049

      参考資料

      参考資料の概要Pub Med ID
      Isocitrate dehydrogenase mutation is frequently observed in giant cell tumor of bone.
      Kato Kaneko, M; Liu, X; Oki, H; Ogasawara, S; Nakamura, T; Saidoh, N; Tsujimoto, Y; Matsuyama, Y; Uruno, A; Sugawara, M; Tsuchiya, T; Yamakawa, M; Yamamoto, M; Takagi, M; Kato, Y
      Cancer science  105  744-8  2014

      概要を表示する
      24898068 24898068
      Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations.
      Kato Kaneko, M; Ogasawara, S; Kato, Y
      The Tohoku journal of experimental medicine  230  103-9  2013

      概要を表示する
      23782684 23782684
      Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi-specific monoclonal antibody MsMab-1.
      Liu, X; Kato, Y; Kaneko, MK; Sugawara, M; Ogasawara, S; Tsujimoto, Y; Naganuma, Y; Yamakawa, M; Tsuchiya, T; Takagi, M
      Cancer medicine  2  803-14  2013

      概要を表示する
      24403254 24403254

      技術情報

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