ABF1820-25UL Anti-Influenza A nucleoprotein

新着ABF1820-25UL
25 µL  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Virus IF, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABF1820-25UL
      DescriptionAnti-Influenza A nucleoprotein
      Alternate Names
      • Nucleoprotein
      • Nucleocapsid protein
      • Protein N
      Background InformationNucleoprotein (UniProt: Q9WLG4; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene in Influenza A virus (strain A/Victoria/3/1975 H3N2). The genomes of all negative-strand RNA viruses are coated by the viral nucleoprotein. Together with the viral RNA-dependent RNA polymerase, these ribonucleoprotein complexes form the minimal functional unit for all viral transcription and replication. Influenza A virus has a negative-sense RNA genome that encodes a single-strand RNA-binding nucleoprotein. The primary function of the nucleoprotein is to encapsidate the virus genome for the purposes of RNA transcription, replication, and packaging. It is considered to be a critical factor in the viral infectious cycle in switching influenza virus RNA synthesis from transcription mode to replication mode. It also functions as a key adapter molecule between virus and host cell processes. It achieves this through its ability to interact with a wide variety of viral and cellular macromolecules, including RNA, itself, two subunits of the viral RNA-dependent RNA polymerase and the viral matrix protein. Nuceloprotein can also interact with cellular polypeptides, including actin, components of the nuclear import and export apparatus and a nuclear RNA helicase. Influenza A nucleoprotein has an unconventional nuclear localization signal (NLS) (aa 1-18) and a bipartite NLS (aa 1980216). (Ref.: Portela, A., and Digard, P. (2002). J. Gen. Virol. 83(4); 723-34; de Lucas, S., et al. (2010). J. Virol. 84(15); 7603-7612).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum in 0.05% sodium azide.
      Applications
      ApplicationAnti-Influenza A nucleoprotein, Cat. No. ABF1820, is a rabbit polyclonal antibody that detects influenza virus nucleoprotein and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

      Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells were infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

      Western Blotting Analysis: A representative lot detected Influenza A nucleoprotein in Western Blotting applications (Jorba, N., et. al. (2009). PLoS Pathog. 5(5):e1000462; Lukarsk, M., et. al. (2017). Nature. 541(7635):117-121; Tripathi, S., et. al. (2015). Cell Host Microbe. 18(6):723-35).

      Immunofluorescence Analysis: A representative lot detected Influenza A nucleoprotein in Immunofluorescence applications (Marcos-Villar, L., et. al. (2018). Sci Rep. 8(1):1230; Rodriguez-Fandsen, A., et. al. (2016). Sci Rep. 6:20744).
      Biological Information
      ImmunogenHig-tagged full length recombinant nucleoprotein from Influenza A virus.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects influenza A virus nucleoprotein in virus infected cells.
      IsotypeIgG
      Species Reactivity
      • Virus
      Species Reactivity NoteInfluenza A Virus.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NP
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~56 kDa observed; 56.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cells infected with A/Victoria/3/75 strain of influenza virus.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Influenza A nucleoprotein in A549 cells infected with A/Victoria/3/75 strain of influenza virus.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications