AB1839 | Anti-Interleukin-6 Antibody

AB1839
100 µL  
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      概要

      Replacement Information

      Special Offers[海外情報]

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Sh ELISA, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1839
      Replaces04-1095
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Interleukin-6 Antibody
      Alternate Names
      • IL-6
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Liquid containing 0.1% sodium azide.
      Applications
      ApplicationAnti-Interleukin-6 Antibody is an antibody against Interleukin-6 for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesDetecting antibody in ELISA, using monoclonal anti-ovine IL-6 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.

      Western Blot

      Optimal working dilutions must be determined by the end user.

      Suggested ELISA Protocol For Ovine IL-6

      1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.

      2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

      3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

      4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

      5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

      6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulphuric acid.

      Notes:

      The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

      Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

      Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
      Biological Information
      ImmunogenRecombinant ovine IL-6
      HostRabbit
      SpecificityReacts with ovine IL-6. No reactivity detected to recombinant ovine IL-1beta, IL-8, MCP or TNFalpha.
      Species Reactivity
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL6
      • HSF
      • BSF-2
      • HGF
      • IL-6
      • BSF2
      • CDF
      • IFNB2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
      SIZE: 212 amino acids; 23718 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: N- and O-glycosylated.
      DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS.
      SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      試験成績書(CoA)

      タイトルロット番号
      RABBIT ANTI-OVINE IL-6 POLYCLONAL ANTIBODY - 2468793 2468793
      RABBIT ANTI-OVINE IL-6 - 2537437 2537437
      RABBIT ANTI-OVINE IL-6 -2752716 2752716
      RABBIT ANTI-OVINE IL-6 POLYCLONAL ANTIBODY - 2224370 2224370
      RABBIT ANTI-OVINE IL-6 POLYCLONAL ANTIBODY - 2377667 2377667

      参考資料

      参考資料の概要アプリケーションPub Med ID
      Oral, nasal and pharyngeal exposure to lipopolysaccharide causes a fetal inflammatory response in sheep.
      Maneenil, G; Kemp, MW; Kannan, PS; Kramer, BW; Saito, M; Newnham, JP; Jobe, AH; Kallapur, SG
      PloS one  10  e0119281  2015

      概要を表示する
      25793992 25793992
      Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep.
      Snyder, CC; Wolfe, KB; Gisslen, T; Knox, CL; Kemp, MW; Kramer, BW; Newnham, JP; Jobe, AH; Kallapur, SG
      American journal of obstetrics and gynecology  208  399.e1-8  2013

      概要を表示する
      ELISA23410690 23410690
      Modulation of lipopolysaccharide-induced chorioamnionitis in fetal sheep by maternal betamethasone.
      Wolfe, KB; Snyder, CC; Gisslen, T; Kemp, MW; Newnham, JP; Kramer, BW; Jobe, AH; Kallapur, S
      Reproductive sciences (Thousand Oaks, Calif.)  20  1447-54  2013

      概要を表示する
      ELISA23653388 23653388
      Selective exposure of the fetal lung and skin/amnion (but not gastro-intestinal tract) to LPS elicits acute systemic inflammation in fetal sheep.
      Kemp, MW; Kannan, PS; Saito, M; Newnham, JP; Cox, T; Jobe, AH; Kramer, BW; Kallapur, SG
      PloS one  8  e63355  2013

      概要を表示する
      23691033 23691033
      Chronic fetal exposure to Ureaplasma parvum suppresses innate immune responses in sheep.
      Kallapur, SG; Kramer, BW; Knox, CL; Berry, CA; Collins, JJ; Kemp, MW; Nitsos, I; Polglase, GR; Robinson, J; Hillman, NH; Newnham, JP; Chougnet, C; Jobe, AH
      Journal of immunology (Baltimore, Md. : 1950)  187  2688-95  2011

      概要を表示する
      21784974 21784974
      Post-natal Changes in Testicular Concentrations of Interleukin-1 Alpha and Beta and Interleukin-6 during Sexual Maturation in Bulls.
      Bagu ET, Gordon JR, Rawlings NC
      Reprod Domest Anim  2009

      概要を表示する
      19144032 19144032
      Progesterone improves acute recovery after traumatic brain injury in the aged rat.
      Sarah M Cutler,Milos Cekic,Darren M Miller,Bushra Wali,Jacob W VanLandingham,Donald G Stein
      Journal of neurotrauma  24  2007

      概要を表示する
      17892409 17892409
      Induction of interleukin-6 and interleukin-12 in bovine B lymphocytes, monocytes, and macrophages by a CpG oligodeoxynucleotide (ODN 2059) containing the GTCGTT motif.
      Y Zhang, L K Shoda, K A Brayton, D M Estes, G H Palmer, W C Brown
      Journal of interferon cytokine research : the official journal of the International Society for Interferon and Cytokine Research  21  871-81  2001

      概要を表示する
      11711000 11711000

      データシート

      タイトル
      Anti-Interleukin-6 - Data Sheet

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies