MAB1004 | Anti-Interleukin-6 Antibody, clone 4B6

MAB1004
250 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Sh ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1004
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Interleukin-6 Antibody, clone 4B6
      Alternate Names
      • IL-6
      References
      Product Information
      FormatPurified
      PresentationLiquid in PBS containing 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Interleukin-6 Antibody, clone 4B6 is validated for use in ELISA for the detection of Interleukin-6.
      Key Applications
      • ELISA
      Application NotesCapture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol.

      Optimal working dilutions must be determined by the end user.

      Suggested ELISA Protocol For Ovine IL-6

      1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.

      2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

      3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

      4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

      5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

      6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.

      Notes:

      The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

      Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

      Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
      Biological Information
      ImmunogenRecombinant ovine IL-6
      Clone4B6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with ovine IL-6. No reactivity detected to recombinant ovine IL-1beta, IL-8, MCP or TNFalpha.

      SPECIES REACTIVITY:

      The antibody is likely to react with caprine (goat) IL-6.
      IsotypeIgG1
      Species Reactivity
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL6
      • IL-6
      • HSF
      • BSF2
      • BSF-2
      • CDF
      • IFNB2
      • HGF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
      SIZE: 212 amino acids; 23718 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: N- and O-glycosylated.
      DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS.
      SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      試験成績書(CoA)

      タイトルロット番号
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY - 2455868 2455868
      MOUSE ANTI-OVINE IL-6 -2522552 2522552
      MOUSE ANTI-OVINE IL-6 -2722612 2722612
      MOUSE ANTI-OVINE IL-6 -2753261 2753261
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY 3087151
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY 3011855
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY 3031649
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY - 2090942 2090942
      MOUSE ANTI-OVINE IL-6 MONOCLONAL ANTIBODY - 2322041 2322041
      MOUSE ANTI-OVINE IL-8 -2751190 2751190

      参考資料

      参考資料の概要アプリケーションPub Med ID
      Oral, nasal and pharyngeal exposure to lipopolysaccharide causes a fetal inflammatory response in sheep.
      Maneenil, G; Kemp, MW; Kannan, PS; Kramer, BW; Saito, M; Newnham, JP; Jobe, AH; Kallapur, SG
      PloS one  10  e0119281  2015

      概要を表示する
      25793992 25793992
      Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep.
      Snyder, CC; Wolfe, KB; Gisslen, T; Knox, CL; Kemp, MW; Kramer, BW; Newnham, JP; Jobe, AH; Kallapur, SG
      American journal of obstetrics and gynecology  208  399.e1-8  2013

      概要を表示する
      ELISA23410690 23410690
      Modulation of lipopolysaccharide-induced chorioamnionitis in fetal sheep by maternal betamethasone.
      Wolfe, KB; Snyder, CC; Gisslen, T; Kemp, MW; Newnham, JP; Kramer, BW; Jobe, AH; Kallapur, S
      Reproductive sciences (Thousand Oaks, Calif.)  20  1447-54  2013

      概要を表示する
      ELISA23653388 23653388
      Selective exposure of the fetal lung and skin/amnion (but not gastro-intestinal tract) to LPS elicits acute systemic inflammation in fetal sheep.
      Kemp, MW; Kannan, PS; Saito, M; Newnham, JP; Cox, T; Jobe, AH; Kramer, BW; Kallapur, SG
      PloS one  8  e63355  2013

      概要を表示する
      23691033 23691033
      Regional pulmonary inflammation in an endotoxemic ovine acute lung injury model.
      A Fernandez-Bustamante,R B Easley,M Fuld,D Mulreany,D Chon,J F Lewis,B A Simon
      Respiratory physiology & neurobiology  183  2012

      概要を表示する
      22728442 22728442
      The impact of intermittent umbilical cord occlusions on the inflammatory response in pre-term fetal sheep.
      Andrew P Prout,Martin G Frasch,Ruud Veldhuizen,Rob Hammond,Brad Matushewski,Bryan S Richardson
      PloS one  7  2012

      概要を表示する
      22745702 22745702
      Interleukin-1 in lipopolysaccharide induced chorioamnionitis in the fetal sheep.
      Clare A Berry,Ilias Nitsos,Noah H Hillman,J Jane Pillow,Graeme R Polglase,Boris W Kramer,Matthew W Kemp,John P Newnham,Alan H Jobe,Suhas G Kallapur
      Reproductive sciences (Thousand Oaks, Calif.)  18  2011

      概要を表示する
      21493953 21493953
      Chronic fetal exposure to Ureaplasma parvum suppresses innate immune responses in sheep.
      Kallapur, SG; Kramer, BW; Knox, CL; Berry, CA; Collins, JJ; Kemp, MW; Nitsos, I; Polglase, GR; Robinson, J; Hillman, NH; Newnham, JP; Chougnet, C; Jobe, AH
      Journal of immunology (Baltimore, Md. : 1950)  187  2688-95  2011

      概要を表示する
      21784974 21784974
      Systemic and cerebral inflammatory response to umbilical cord occlusions with worsening acidosis in the ovine fetus.
      Andrew P Prout,Martin G Frasch,Ruud A W Veldhuizen,Rob Hammond,Michael G Ross,Bryan S Richardson
      American journal of obstetrics and gynecology  202  2010

      概要を表示する
      19889382 19889382

      データシート

      タイトル
      Anti-Interleukin-6, clone 4B6 - Data Sheet

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies