ABE1322-100UG | Anti-MBD5

新着ABE1322-100UG
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, IH(P), WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE1322-100UG
      DescriptionAnti-MBD5
      Alternate Names
      • Methyl-CpG-binding domain protein 5
      • Methyl-CpG-binding protein MBD
      Background InformationMethyl-CpG-binding domain protein 5 (UniProt: Q9P267; also known as Methyl-CpG-binding protein MBD5) is encoded by the MBD5 (also known as KIAA1461) gene (Gene ID: 55777) in human. MBD proteins play a role in diverse processes, including chromatin remodeling, DNA damage repair, histone methylation, and X chromosome inactivation. MBD5 is detected in heart, placenta, liver, skeletal muscle, kidney, and pancreas. It binds to heterochromatin, but does not interact with either methylated or unmethylated DNA (in vitro). It contains a MBD domain (aa 11-81) and a PWWP (Pro-Trp-Trp-Pro) domain (aa 1385-1447). Both MBD and PWWP domains are shown to be essential for chromocentric localization. Two isoforms of MBD5 have been reported that are produced by alternative splicing. However, only the isoform 1 associates with pericentric heterochromatin. Mutations in MBD5 gene are known to cause autosomal dominant mental retardation, which is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. (Ref.: Camarena, V et al., (2014). EMBO Mol. Med. 6(8); 1003 1015).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MBD5, Cat. No. ABE1322, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD5 in human pancreas and human cervical cancer tissue sections

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD5 in HeLa, A431, and HEK293 cells.

      Western Blotting Analysis: 2 µg/mL from a representative lot detected MBD5 in human liver tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Methyl-CpG-binding domain protein 5 (MBD5).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects isoform 1 of human Methyl-CpG-binding domain protein 5. It targets an epitope within 16 amino acids fromt the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MBD5
      • KIAA1461
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~160 kDa observed; 159.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human prostate tissue sections.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MBD5 in human prostate tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications