ABN2181-25UG Anti-NCAM-L1

新着ABN2181-25UG
25 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IF, IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberABN2181-25UG
      DescriptionAnti-NCAM-L1
      Alternate Names
      • Neural cell adhesion molecule L1-like protein
      • L1 adhesion molecule
      • Cell adhesion molecule with homology to L1CAM
      • Chl1-like protein
      • Close homolog of L1
      Background InformationNeural cell adhesion molecule L1-like protein (UniProt: P70232; also known as Cell adhesion molecule with homology to L1CAM, NCAM-L1, Chl1-like protein, Close homolog of L1) is encoded by the Chl1 (also known as Call) gene (Gene ID: in murine species. NCAM-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It is expressed in neurons and glial cells in the brain and the spinal cord and has also been detected in the retina and the optic nerve. It is not detectable in mouse forebrain at E11 and becomes weakly detectable at E13 with highest detection at E18 to postnatal day 7. It is down-regulated at postnatal day 15 and is further reduced in four-week-old animals. NCAM-L1 is heavily N-glycosylated and contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1. NCAM-L1 contains an extracellular domain (aa 26-1083), a transmembrane domain (aa 1084-1104), and a cytoplasmic domain (aa 1105-1209). It also has six Ig-like C2 type domains and four fibronectin type -III domains. NCAM-L1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. It plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-NCAM-L1, Cat. No. ABN2181, is a highly specific rabbit polyclonal antibody that targets Neural cell adhesion molecule L1 and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected NCAM-L1 in Western Blotting applications (Schmidt, H., et. al. (2002). J Cell Biol. 159(3):489-98; Alberts, P., et. al. (2003). Mol Biol Cell. 14(10):4207-20).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated NCAM-L1 in Immunoprecipitation applications (Patel, K., et. al. (1991). Hybridoma. 10(4):481-91).

      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NCAM-L1 in crude membrane fractions of postnatal day 10 brain extracts (Courtesy of Fritz G. Rathjen from Max-Delbrück-Center, Berlin Germany).

      Immunofluorescence Analysis: A representative lot detected NCAM-L1 in Immunofluorescence applications (Ter-Avetisyan, G., et. al. (2014). J Neurosci. 34(3):737-47; Abe, P., et. al. (2015). J Neurosci. 35(38):13053-63).
      Biological Information
      ImmunogenPurified full-length murine Neural cell adhesion molecule L1-like protein (NCAM-L1).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects NCAM-L1 protein in mouse and rat.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Chl1
      • Call
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~220 kDa observed; 135.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Wild type & knock out P10 mouse brain extract crude membrane.

      Western Blotting Analysis: 2 µg/mL of this antibody detected NCAM-L1 in crude membrane extact from brain of Wild type, but not in NCAM-L1 knock out P10 mouse.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications