04-1557-25UL | Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal)

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25 µL  
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      Description
      Catalogue Number04-1557-25UL
      DescriptionAnti-SIRT1 Antibody, clone 10E4 (mouse monoclonal)
      Alternate Names
      • SIR2-like protein 1
      • sir2-like 1
      • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
      • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
      • sirtuin 1
      • sirtuin type 1
      Background InformationSir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation: Reported by independent researcher using representative lot.
      Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
      Immunohistochemistry: Reported by independent researcher using representative lot.
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human SIRT1.
      EpitopeUnknown
      Clone10-E-4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody recognizes SIRT1.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
      Gene Symbol
      • SIRT1
      • SIR2L1
      • hSIR2
      • hSIRT1
      • SIR2alpha
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2- mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.

      COFACTOR: Binds 1 zinc ion per subunit (By similarity).

      SIZE: 747 amino acids; 81681 Da

      SUBUNIT: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity). Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML.

      SUBCELLULAR LOCATION: Nucleus. Note=Recruited to the nuclear bodies via its interaction with PML.TISSUE SPECIFICITY: Widely expressed.

      SIMILARITY: Belongs to the sirtuin family. & Contains 1 deacetylase sirtuin-type domain.

      MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.
      Molecular Weight~ 110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications

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