ABN2264-50UL | Anti-SNTF (cleaved spectrin)

新着ABN2264-50UL
50 µL  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Porcine ELISA, IHC, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABN2264-50UL
      DescriptionAnti-SNTF (cleaved spectrin)
      Alternate Names
      • Spectrin alpha chain non-erythrocytic 1
      • Alpha-II spectrin
      • Fodrin alpha chain
      • Spectrin non-erythroid alpha subunit
      • Calpain-derived alpha-II spectrin N-terminal fragment
      Background InformationSpectrin alpha chain, non-erythrocytic 1 (UniProt: Q13813; also known as Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit) is encoded by the SPTAN1 (also known as NEAS, SPTA2) gene (Gene ID: 6709) in human. Non-erythroid Spectrin plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. Alpha II-spectrin is a structural protein abundant in neurons of the central nervous system and is cleaved into signature fragments by proteases involved in necrotic and apoptotic cell death. It is one of the primary targets cleaved by activated caspases during apoptosis. The full-length protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa. Alpha II- spectrin is involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. It has two calcium-binding regions (aa 2336-2347 and 2379-2390). Its mu-calpain cleavage site is localized at Tyr-Gly (aa 1176-1177). Phosphorylation of Tyrosine 1176 is shown to reduce sensitivity to cleavage by calpain in vitro. Plasma levels of calpain-cleaved Alpha II-Spectrin, N-terminal fragment (SNTF) serve as a useful marker for mild to severe traumatic brain injury and SNTF immunostaining can also reveal previously undetected traumatic brain injury. Mutations in SPTAN1 gene are known to cause early infantile epileptic encephalopathy that is characterized by seizures associated with hypsarrhythmia, profound mental retardation with lack of visual attention and speech development. (Ref.: Siman, R., et al. (2013). Front. Neurol. 4; Article 190; Johnson VE., et al (2016). Acta Neuropathol. 131(1); 115-135).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-SNTF (cleaved spectrin), Cat. No. ABN2264, is a highly specific rabbit polyclonal antibody that targets cleaved Spectrin alpha chain, non-erythrocytic 1 and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected SNTF (cleaved spectrin) in swine and human brain sections (Johnson, V.E., et. al. (2016). Acta Neuropathol. 131(1):115-35).

      Western Blotting Analysis: A representative lot detected SNTF (cleaved spectrin) in Western Blotting applications (Roberts-Lewis, J.M., et. al. (1994). J Neurosci. 14(6):3934-44).

      Enzyme Immunoassay Analysis (ELISA): A representative lot detected SNTF (cleaved spectrin) in detection antibody for human serum samples (Siman, R., et. al. (2013). Front Neurol. 4:190).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to calpain-generated neoepitope in SNTF (CAQQEVY).


      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects calpain-generated neoepitope in SNTF. It targets a neoepitope of 6 amino acids at the carboxyl-end of the stable calpain-derived alpha-spectrin.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Porcine
      Species Reactivity NoteHuman, Mouse, Porcine. Predicted to react with Rat and Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPTAN1
      • NEAS
      • SPTA2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~160 kDa observed. Calculated molecular weight of full length protein is 284.54 kDa. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain extract incubated with calcium chloride to activate calpain.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Spectrin alpha chain, non-erythrocytic 1 in Mouse Brain extract incubated with calcium chloride to activate Calpain.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications