AB5603-25UG | Anti-Sox2 (rabbit polyclonal)

25 μg  
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      Catalogue NumberAB5603-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sox2 (rabbit polyclonal)
      Alternate Names
      • Transcription factor SOX-2
      • Sex determining region Y-box 2
      • SRY-related HMG-box gene 2
      • Transcription factor SOX2
      Background InformationTranscription factor SOX-2 (UniProt P48431; also known as Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2) is encoded by the SOX2 (also known as ANOP3, MCOPS3) gene (Gene ID 6657) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences coding the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at at least 40 genomic loci and at least 30 SOX genes have been identified. Sox2 plays an essential role in maintaining the pluripotency and self-renewal of undifferentiated embryonic stem cells.
      Product Information
      FormatAffinity Purified
      • Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
        Immunocytochemistry: Human embryonic stem cells (H9 line)
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Sox2 Antibody is an antibody against Sox2 for use in IC & WB with more than 60 product citations.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
      Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
      Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
      Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson's disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
      Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
      Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
      Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
      Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.
      EpitopeC-terminal region.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis antibody recognizes Sox2 C-terminal region.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOX2
      • ANOP3
      • MCOPS3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mESC lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sox2 in 10 µg of mESC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information