ABS1679 | Anti-VPS28

200 µL  
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      Replacement Information


      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, WB Rb Purified Polyclonal Antibody
      Catalogue NumberABS1679
      Alternate Names
      • Vacuolar protein sorting-associated protein 28 homolog
      • H-Vps28
      • ESCRT-I complex subunit VPS28
      Background InformationVacuolar protein sorting-associated protein 28 homolog (UniProt: Q9UK41; also known as H-Vps28, ESCRT-I complex subunit VPS28) is encoded by the VPS28 gene (Gene ID: 51160) in human. VPS28 is a component of the Endosomal Sorting Complex Required for Transport (ESCRT)-I complex that regulates the vesicular trafficking process. This complex forms the machinery driving protein sorting from endosomes to lysosomes. It is located in the cell membrane and late endosome membrane. Two isoforms of VPS28 have been described that are generated by alternative splicing. ESCRT complexes play a vital role in receptor down-regulation, lysosome biogenesis, and budding of HIV. In humans, ESCRT-I complex consists of TSG101, VPS28, and one of four potential human VPS37 homologues. The main role of ESCRT-I is to recognize ubiquitinated cargo via the UEV domain of the VPS23/TSG101 subunit. The N-terminal domain of VPS28 contains a conformationally dynamic hydrophobic binding site on its surface. The C-terminal domain of VPS28 is reported to bind the ESCRT-II complex. (Ref.: Kostelansky, MS et al. (2006). Cell 125(1); 113-126).
      Product Information
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-VPS28, Cat. No. ABS1679, is a rabbit polyclonal antibody that detects ESCRT-I complex subunit VPS28 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected recombinant VPS28 protein and VPS28 in cell extracts (Stuchell, M.D., et. al. (2004). J Biol Chem. 279(34):36059-71).

      Immunofluorescence Analysis: A representative lot detected VPS28 in HEK293T cells transfected with VPS28 (Silvestri, L.S., et. al. (2007). J Infect Dis. 196 Suppl 2:S264-70).
      Biological Information
      ImmunogenA full-length recombinant ESCRT-I complex subunit VPS28 (VPS28).
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis rabbit polyclonal antibody detects ESCRT-I complex subunit VPS28 in human cells.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VPS28
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~51 kDa observed; 25.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GST-VPS28 recombinant protein.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant GST-VPS28 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information