IPVH15150 イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 15 x 15 cm シート

IPVH15150
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      概要

      Replacement Information
      Replacement InformationINCP 151 50 (Protein Applications)

      主要スペック表

      主要アプリケーション
      WB
      説明
      カタログ番号IPVH15150
      ReplacesINCP 151 50 (タンパク質用途)
      製品名
      • Immobilon
      説明イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 15 x 15 cm シート
      背景情報Overview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      参考資料
      製品情報
      検出方法Chemiluminescent(化学発光検出法) Chromogenic(発色検出法) Radioactive(ラジオアイソトープ法)
      適合染料
      • クマシーブリリアントブルー
      • アミドブラック
      • インディアインク
      • ポンソーSレッド
      • 金コロイド
      • CPTS
      • トルイジンブルー
      • トランスイルミネーション
      • Sypro® ruby
      フィルターコードIPVH
      フィルターカラー白色
      アプリケーション
      アプリケーションウエスタンブロッティング, バインディングアッセイ, アミノ酸分析, N-末端プロテインシークエンシング, ドット/スロットブロッティング, glycoprotein visualization, lipopolysaccharide analysis
      主要アプリケーション
      • Western Blotting
      生体情報
      主要結合の仕組み静電気的結合, 疎水性相互作用
      BSA吸着215 µg/cm²
      ヤギIgG吸着294 µg/cm²
      インスリン吸着160 µg/cm²
      湿潤性疎水性
      物理化学情報
      ポアサイズ0.45 µm
      寸法
      フィルター表面プレーン
      フィルター寸法15 cm x 15 cm
      材料情報
      材質
      • 疎水性PVDF
      有害性情報
      GHSに関する安全性情報
      安全性情報
      製品使用について
      保管および輸送に関する情報
      パッケージ情報
      数量10
      輸配送情報
      補足情報
      規格

      Documentation

      イモビロン(Immobilon)-P メンブレン, PVDF, 0.45 µm, 15 x 15 cm シート (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      参考資料

      参考資料の概要アプリケーションPub Med ID
      A role for a CXCR2/phosphatidylinositol 3-kinase gamma signaling axis in acute and chronic vascular permeability.
      Gavard, Julie, et al.
      Mol. Cell. Biol., 29: 2469-80 (2009)  2009

      概要を表示する
      Immunoblotting (Western)19255141 19255141
      Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
      Luo S., Wehr N.B., Levine R.L.
      Analytical Biochemistry:350 (2006):233-238  2006

      Immunoblotting (Western)
      Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology
      Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M.
      Neuroscience 120 (2003) 295-705  2003

      Western Blotting

      カタログ

      タイトル
      Western Blotting Tools

      技術情報

      タイトル
      Protein Blotting Handbook: 6th Edition (Merck)
      Protein Dot Blotting using Immobilon-P

      データシート

      タイトル
      Immobilon Transfer Membranes: For superior protein and nucleic acid blots

      ユーザーガイド

      タイトル
      Immobilon -P Blotting Sandwiches
      Immobilon®-P Transfer Membrane User Guide