SCT012 | LuminiCell Tracker™ 540- Vascular Labeling Kit

SCT012
1 Kit  
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      概要

      Replacement Information
      Description
      Catalogue NumberSCT012
      DescriptionLuminiCell Tracker™ 540- Vascular Labeling Kit
      OverviewAbsorbance Maximum: 423nm
      Emisson Maximum: 540nm

      Suggested Filters:
      Excitation: 405/458/488nm
      Emisson: 480-560nm
      Alternate Names
      • AIEDots Cell Tracker Dye
      • AIE Live Cell Fluorescent Dye
      • Fluorescent Cell Tracking Dye
      • Live Cell Fluorescent Nanoparticles
      Background InformationLong-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers™ are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers™ have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.

      The Luminicell Tracker™-540 Vascular Labeling Kit contain green fluorescent AIEDots nanoparticles without TAT sequences. These nanoparticles can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

      Features and Benefits

      ● Brighter: 10X brighter than other cell labeling technologies
      ● Photostable: 3X longer fluorescence without signal quenching
      ● Biocompatible: Non-toxic organic nanoparticles intended for biological applications
      ● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

      References

      1) Liu B, Tang BZ et al. Photostable fluorescent organic dots with aggregation-induced emission (AIE dots) for noninvasive long-term cell tracing. Sci Rep. 2013;3:1150.
      2) Kang Y et al. Long-Term Tracking Mesenchymal Stem Cell Differentiation with Photostable Fluorescent Nanoparticles. ACS Appl Mater Interfaces. 2016 May 18;8(19):11925-33.
      3) Tang, B. Aggregation-Induced Emission: Together We Shine, United We Soar! Chem Rev. 2015 Nov 11;115(21):11718-940.
      References
      Product Information
      Presentation500nM in 0.5ml PBS, pH 7.4
      Applications
      ApplicationBiocompatible organic fluorescent nanoparticles (AIEDots) that can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.
      Key Applications
      • Cell Culture
      • Fluorescence
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAbsorbance: 422+-5nM
      Concentration: 180-220nM
      Fluorescence: 540+/- 10nm
      Quantum Yield: ≥50%
      Brightness at 540nM: ≥3X10^7 M-1cm-1
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

      Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker™ three times for 1 min each before use.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      試験成績書(CoA)

      タイトルロット番号
      LuminiCell Tracker™ 540- Cell Labeling Kit VP1806140

      データシート

      タイトル
      LuminiCell Tracker™ 540- Vascular Labeling Kit

      Posters

      タイトル
      A novel biocompatible fluorescent nanoparticle enables enhanced live cell tagging and tracking of cancer cells
      Cancer stem cell proliferation in human prostate and breast cancer cell lines utilizing a new defined serum-free 3D spheroid media