Chromolith® HPLC Column Coupler

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Perfect Combinations for Complex Mixtures

Chromolith® HPLC column couplerThe Chromolith® HPLC column coupler is designed for combining several monolithic columns together in order to further increase separation efficiency and column performance. The combination can result in a theoretical plate count that is significantly higher than that of particulate columns. At the same time, pressure is kept well below the HPLC system limit.

The superior column performance achieved by using the Chromolith® HPLC column coupler allows you to solve highly critical separation problems in which resolution is a limiting factor. This makes column coupling perfect for chromatographic separations of hitherto non-separable, complex mixtures.

Typical Column Efficiency Using the Chromolith® HPLC Column Coupler

The table below shows a comparison between Chromolith® HPLC columns and particulate columns. The combination of just two Chromolith® Performance RP-18 endcapped columns yields a separation efficiency of 19,000 theoretical plates per column, which is usually the maximum for particulate columns.

Column
Length [mm]
Pressure [bar]
Plate number per column [Anthracene]

Chromolith® Performance 1x

100 30 10,000

Chromolith® Performance 2x

200 60 19,000

Chromolith® Performance 3x

300 90 27,000

Chromolith® Performance 4x

400 120 35,000
Chromolith® Performance 5x 500 150 41,000
Particulate column
(5 μm)
250 220 18,500
Particulate column (3.5 μm) 150 400 19,000

Separation of 7 Alkenes Using Coupled Chromolith® Performance RP-18e Columns (81,000 Plates at 85 Bar Pressure)

Column Column 10 columns of Chromolith® Performance RP-18e, 100-4.6 mm
Mobile phase 80 /20 Acetonitrile/water  
Flow rate 1 mL/min
Detection UV 254 nm
Temperature ambient
Injection volume 10 μl
Sample

1. Thiourea
2. Benzene
3. Toluene
4. Ethylbenzene
5. Propylbenzene
6. Butylbenzene
7. Phenylbenzene

Separation of 7 alkenes using coupled Chromolith®

Separation of 11 Steroids Using Coupled Chromolith® HighResolution RP-18e Columns

Column Chromolith® HighResolution RP-18e 2 x 100-4.6 mm / 1 x 50-4.6 mm
Mobile phase ACN / water
Gradient Time/min %A %B
0 55 45
2 95 5
15 95 5
Flow rate 1 mL/min
Pressure 30 - 68 bar
Detection UV 240 nm
LC System LaChrom® L7000
Vol. detector cell 16 μL
Temperature ambient
Injection volume 10 μl
Sample

1. Fluoxymesterone
2. Boldenone
3. Methandrostenolone
4. Testosterone
5. Methyltestosterone
6. Boldenone acetate
7. Testosterone acetate
8. Nandrolone propionate
9. Testosterone propionate
10. Nandrolone phenylpropionate
11. Testosterone isocaproate

Separation of 11 steroids using coupled Chromolith®

 
 
 
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Chromolith® WidePore 300 HPLC Columns

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Chromolith® WidePore 300 HPLC Columns

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