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AB5054-K | Anti-Calretinin (1mL)

AB5054-K
Bulk packaging. Please inquire.  
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      개요

      Replacement Information
      Description
      Catalogue NumberAB5054-K
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Calretinin (1mL)
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Calretinin
      Background InformationCalretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.
      References
      Product Information
      FormatSerum
      Control
      • Rat brain cortex, Mesothelioma tissue, Human cerebellum.
      PresentationRabbit polyclonal serum contains no preservative.
      Applications
      ApplicationThis Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot:
      1:1,000-1:2,000 of a previous lot was used.

      Immunohistochemistry:
      1:1,000-1:10,000
      Antibody works in paraffin embedded tissues.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant rat calretinin.
      ConcentrationVariable
      HostRabbit
      SpecificitySpecific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman and rat. Reactivity with other species has not been determined
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.
      Gene Symbol
      • CALB2
      • CAB29
      • CR
      • CAL2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22676 # Calretinin is a calcium-binding protein which is abundant in auditory neurons.
      SIZE: 271 amino acids; 31540 Da
      TISSUE SPECIFICITY: Brain.
      SIMILARITY: SwissProt: P22676 ## Belongs to the calbindin family. & Contains 6 EF-hand domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry(paraffin) Analysis:
      Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
      Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications

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      AB5054 Anti-Calretinin Antibody 가격 및 재고 조회

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      카테고리

      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Neurological Proteins
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Secondary Bulk Antibodies > Other Secondary Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Tumor Markers & Antigens
      Life Science Research > Antibodies and Assays > Primary Antibodies