17-701 EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

17-701
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
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      개요

      Replacement Information

      주요 사양표

      Key Applications
      RIP
      Description
      Catalogue Number17-701
      Brand Family Upstate
      Trade Name
      • EZ-Magna RIP
      • Upstate
      DescriptionEZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Positive and negative controls
      Alternate Names
      • Magnetic RIP kit
      Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      References
      Product Information
      Components
      • Magnetic Beads Protein A/G
      • RIP Wash Buffer
      • RIP Lysis Buffer
      • 0.5 M EDTA
      • 10% SDS
      • Salt Solution I
      • Salt Solution II
      • Precipitate Enhancer
      • Normal Mouse IgG
      • Rabbit IgG Purified
      • Protease Inhibitor Cocktail 200X
      • RNase Inhibitor
      • Proteinase K (10 mg/mL)
      • Nuclease free water
      • Positive Control Antibody (Anti-SNRNP70)
      • Control Primers
      PresentationThree boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Includes positive control antibody and control primers.
      Applications
      ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers included.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size12 assays
      Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit MSDS

      타이틀

      물질안전보건자료(MSDS) 

      References

      Reference overviewApplicationPub Med ID
      MiR-155-5p controls colon cancer cell migration via post-transcriptional regulation of Human Antigen R (HuR).
      Al-Haidari, A; Algaber, A; Madhi, R; Syk, I; Thorlacius, H
      Cancer Lett  421  145-151  2018

      요약 표시
      29471005 29471005
      MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer.
      Wang, H; Wang, L; Zhang, G; Lu, C; Chu, H; Yang, R; Zhao, G
      Oncotarget  9  7501-7512  2018

      요약 표시
      29484127 29484127
      Long Non-coding RNA LINC00339 Stimulates Glioma Vasculogenic Mimicry Formation by Regulating the miR-539-5p/TWIST1/MMPs Axis.
      Guo, J; Cai, H; Liu, X; Zheng, J; Liu, Y; Gong, W; Chen, J; Xi, Z; Xue, Y
      Mol Ther Nucleic Acids  10  170-186  2018

      요약 표시
      29499931 29499931
      LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein.
      Liang, Y; Chen, X; Wu, Y; Li, J; Zhang, S; Wang, K; Guan, X; Yang, K; Bai, Y
      Cell Death Differ  0  2018

      요약 표시
      29511340 29511340
      Studying the mechanism of PLAGL2 overexpression and its carcinogenic characteristics based on 3'-untranslated region in colorectal cancer.
      Su, C; Li, D; Li, N; Du, Y; Yang, C; Bai, Y; Lin, C; Li, X; Zhang, Y
      Int J Oncol  0  2018

      요약 표시
      29512763 29512763
      miR-296-3p Negatively Regulated by Nicotine Stimulates Cytoplasmic Translocation of c-Myc via MK2 to Suppress Chemotherapy Resistance.
      Deng, X; Liu, Z; Liu, X; Fu, Q; Deng, T; Lu, J; Liu, Y; Liang, Z; Jiang, Q; Cheng, C; Fang, W
      Mol Ther  26  1066-1081  2018

      요약 표시
      29525743 29525743
      LncRNA PVT1 regulates triple-negative breast cancer through KLF5/beta-catenin signaling.
      Tang, J; Li, Y; Sang, Y; Yu, B; Lv, D; Zhang, W; Feng, H
      Oncogene  0  2018

      요약 표시
      29760406 29760406
      Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells.
      Duan, Z; Ping, P; Wang, G; Zhang, X; Sun, F
      Biosci Rep  0  2018

      요약 표시
      29769412 29769412
      LncRNA DICER1-AS1 promotes the proliferation, invasion and autophagy of osteosarcoma cells via miR-30b/ATG5.
      Gu, Z; Hou, Z; Zheng, L; Wang, X; Wu, L; Zhang, C
      Biomed Pharmacother  104  110-118  2018

      요약 표시
      29772430 29772430
      LncRNA TUG1 sponges miR-204-5p to promote osteoblast differentiation through upregulating Runx2 in aortic valve calcification.
      Yu, C; Li, L; Xie, F; Guo, S; Liu, F; Dong, N; Wang, Y
      Cardiovasc Res  114  168-179  2018

      요약 표시
      29016735 29016735

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      User Guides

      Title
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

      관련 제품 및 어플리케이션

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      관련 제품: Brand Facete

      카테고리

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits