|Isolation of measles virus from clinical specimens using B95a and Vero/hSLAM cell-lines. |
Keniscope C, Juliana R, Subri H, Shangari SR, Wan Nor Azlina WA, Hamizah A, Emmi EE, Nor Azlina MD, Norizah I, Chua KB
Med J Malaysia
The clinical presentation of acute measles is normally quite typical, especially in the presence of Koplik's spots, that laboratory test is seldom required to confirm the diagnosis. However, with wide measles vaccination coverage and the extensive use of immunosuppressive chemotherapy, the diagnosis of atypical manifestations of acute measles may require laboratory confirmation. When compared with B95a cell-line, this study shows that the Vero/hSLAM cell-line is sensitive and is recommended for use in the primary isolation of wild-type measles virus from clinical specimens. Throat swab and urine specimens are the clinical specimens of choice and both are recommended for optimal isolation of measles virus from patients suspected of acute measles virus infection.
|Differences in insulin sensitivity and secretory capacity based on OGTT in subjects with impaired glucose regulation. |
Sang Youl Rhee, Mi Kwang Kwon, Byong-Jo Park, Suk Chon, In-Kyung Jeong, Seungjoon Oh, Kyu Jeung Ahn, Ho Yeon Chung, Sung Woon Kim, Jin-Woo Kim, Young Seol Kim, Jeong-Taek Woo
The Korean journal of internal medicine
BACKGROUND: This study examined whether defects in insulin secretion contribute to the development and progression of type 2 diabetes mellitus (T2DM). METHODS: Plasma insulin and glucose were measured after a glucose tolerance test to calculate the insulinogenic index (IGI) and the HOMA-IR Homeostasis model assessment of insulin resistance in subjects with normal glucose tolerance (NGT), prediabetes (preDM, n = 49), and T2DM patients with disease duration 1 year (n = 84), 1 approximately 5 years (n = 45), or 5 years (n = 37). Plasma proinsulin and adiponectin levels were also measured as a parameter of insulin secretion and resistance. RESULTS: The mean HOMA-IR increased and the adiponectin levels decreased relative to the deterioration of glucose tolerance in NGT and preDM subjects. However, differences in the HOMA-IR were not related to disease duration in T2DM subjects. The mean IGI was similar in NGT and preDM subjects, but there were significant deteriorations in IGI relative to the duration of diabetes. CONCLUSIONS: Defects in both insulin sensitivity and insulin secretion contribute to T2DM, but decreased insulin secretion may be more important in the development and progression of T2DM.기사 전문