71345 | Origami™ 2(DE3) Competent Cells - Novagen

71345
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      가격 및 재고여부

      카탈로그 번호재고 정보 패킹 포장 단위 가격 수량
      71345-3CN
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          0.4 ml
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          71345-4CN
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              1 ml
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              Description
              Overview Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
              Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB (StrR, TetR)
              Catalogue Number 71345
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiency 2 × 10⁶ cfu/µg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Do not freeze No
              Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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              Certificates of Analysis

              TitleLot Number
              71345

              Brochure

              Title
              Competent Cell Brochure

              Citations

              타이틀
            • Naur, P., et al. 2007. Procedings of the National Academy of Science 104, 14116.
            • User Protocols

              Title
              TB009 Competent Cells
              TB053 Academic and Non-profit Laboratory Assurance Letter
              TB055 pET System Manual

              관련 제품 및 어플리케이션

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              카테고리

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression