Cell-Cell Interaction

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Human plasmacytoid dendritic cells and myeloid dendritic cells fuse with chronically HIV-infected T cells
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Measuring Immunological Synapse Using the FlowSight Imaging Flow Cytometer
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Merck:/Freestyle/BI-Bioscience/Cell-Analysis/amnis/Amins2-images/cell-cell-interaction2.jpgMany biological responses are initiated through direct cell-cell interactions. Such interactions may result in the recruitment of signaling molecules to the interacting point of contact (such as at the immune synapse), or in transfer of materials between cells, or cellular fusion. These systems are naturally suited for microscopic analysis, and the high speed image acquisition and quantitative image analysis capabilities of Amnis® imaging flow cytometers bring major advances to the study of cell-cell interactions. Single cells, conjugates, and fusion events are easily distinguishable in statistically significant sample sizes, and accumulation of protein at the synapse or transfer of material between two cells readily identified and quantified.

Featured Video

Watch to learn how multispectral imaging in flow can be used to answer specific cell interaction research questions. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging to characterize cell-cell interaction events. Using antigen presenting cells activated with enterotoxin B and incubated with T cells, microscopy in flow can be used to enhance analysis of the immune synapse.

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Ovalbumin Specific T Cells Incubated with Antigen Presenting Cells +/- Ovalbumin Peptide

Ovalbumin-specific accumulation of ADAP at the immune synapse is measured. Quantitation of conjugate formation and representative cell images show that without specific peptide, ADAP (green) is distributed around the T cell. In the presence of specific peptide, ADAP moves to the synapse between the APC and T cell.

Membrane Transfer Between Cells

Transfer of PKH26 labeled cell membrane (red) from antigen presenting cells (APC) to T cells identified by CD3 (green). Cell images show T cell:APC conjugates (gate R8) and single T cells with APC membrane material (gate R3).

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