123900 AKTide-2T

123900
  
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      An optimal peptide substrate for assaying Akt/PKB/Rac-protein kinase activity in vitro. The peptide undergoes phosphorylation at the Ser site (Km = 3.9 µM). Competitively inhibits histone H2B phosphorylation (Ki = 12 µM) by Akt.
      Catalogue Number123900
      Brand Family Calbiochem®
      SynonymsARKRERTYSFGHHA
      References
      ReferencesObata, T., et al. 2000. J. Biol. Chem. 275, 36108.
      Product Information
      FormOff-white solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₄H₁₁₄N₂₈O₂₀
      Chemical formulaC₇₄H₁₁₄N₂₈O₂₀
      Hygroscopic Hygroscopic
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide SequenceH-Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      123900

      Références bibliographiques

      Aperçu de la référence bibliographique
      Obata, T., et al. 2000. J. Biol. Chem. 275, 36108.

      Brochure

      Titre
      Akt
      Biologics 31.1
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-April-2008 RFH
      SynonymsARKRERTYSFGHHA
      DescriptionAn optimal peptide substrate for assaying AKT/PKB/Rac-protein kinase activity in vitro. The peptide undergoes phosphorylation at the Ser site (Km = 3.9 µM). Reported to competitively inhibit histone H2B phospho-rylation (Ki = 12 µM) by AKT.
      FormOff-white solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions
      The reaction mixture (30 µl) contains 20 mM HEPES, pH 7.4, 100 µM ATP with 3 µCi of [γ-32P]-ATP, 10 mM DTT, 10 mM MgCl2, 0.5 mM EGTA, and 50 µM AKTide-2T. Reaction begins with the addition of 0.1 µg of purified AKT. Incubation is carried out at 25°C for designated time periods (5 to 15 min). Reaction is terminated by adding 10 µl of stop solution containing 8 N HCl and 1 mM ATP. Aliquots are then spotted on P81-phosphocellulose paper, washed four times in 0.5% phosphoric acid, and quantified by scintillation counting. Appropriate blanks are run concurrently.
      Chemical formulaC₇₄H₁₁₄N₂₈O₂₀
      Peptide SequenceH-Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala-OH
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesObata, T., et al. 2000. J. Biol. Chem. 275, 36108.