113000 | Acridine Orange - CAS 65-61-2 - Calbiochem

113000
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      Descripción

      Replacement Information

      Tabla espec. clave

      Empirical FormulaCAS #
      C₁₇H₂₀ClN₃ 65-61-2

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US1113000-1GM
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          Frasco de vidrio 1 gm
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          Description
          OverviewA cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
          Catalogue Number113000
          Brand Family Calbiochem®
          SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
          References
          ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
          Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
          Olivier, R. 1995. Methods Enzymol. 251, 270.
          Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
          Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
          Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
          El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
          Lopez, F., et al. 1991. Cytometry 12, 42.
          Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
          Hermansen, D.K., et al. 1989. Cytometry 10, 739.
          Product Information
          CAS number65-61-2
          ATP CompetitiveN
          FormBurnt-orange solid
          Hill FormulaC₁₇H₂₀ClN₃
          Chemical formulaC₁₇H₂₀ClN₃
          Hygroscopic Hygroscopic
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetInteracts with DNA and RNA
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSAR7601000
          Safety Information
          R PhraseR: 20/21/22-40

          Harmful by inhalation, in contact with skin and if swallowed.
          Limited evidence of a carcinogenic effect.
          S PhraseS: 36

          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful & Carcinogenic / Teratogenic
          Storage +15°C to +30°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          113000

          Referencias bibliográficas

          Visión general referencias
          Gonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
          Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
          Olivier, R. 1995. Methods Enzymol. 251, 270.
          Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
          Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
          Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
          El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
          Lopez, F., et al. 1991. Cytometry 12, 42.
          Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
          Hermansen, D.K., et al. 1989. Cytometry 10, 739.

          Folleto

          Cargo
          Caspases and other Apoptosis Related Tools Brochure
          Dyes and Stains Technical Bulletin
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-April-2008 RFH
          SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
          DescriptionA cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
          FormBurnt-orange solid
          CAS number65-61-2
          RTECSAR7601000
          Chemical formulaC₁₇H₂₀ClN₃
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMF, Ethanol, or H₂O (1 mg/ml)
          Storage +15°C to +30°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
          Toxicity Harmful & Carcinogenic / Teratogenic
          ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
          Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
          Olivier, R. 1995. Methods Enzymol. 251, 270.
          Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
          Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
          Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
          El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
          Lopez, F., et al. 1991. Cytometry 12, 42.
          Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
          Hermansen, D.K., et al. 1989. Cytometry 10, 739.