HADCYMAG-61K MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

HADCYMAG-61K
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Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α H Mplex Luminex xMAP
Description
Catalogue NumberHADCYMAG-61K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Adipocyte Magnetic Bead Panel is a 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα.
Background InformationThe role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Adiponectin: Intra-assay <10% CV Inter-assay <15% CV
  • HGF: Intra-assay <10% CV Inter-assay <20% CV
  • IL-1β: Intra-assay <15% CV Inter-assay <20% CV
  • IL-6: Intra-assay <10% CV Inter-assay <20% CV
  • IL-8: Intra-assay <10% CV Inter-assay <15% CV
  • Leptin: Intra-assay <20% CV Inter-assay <25% CV
  • MCP-1: Intra-assay <10% CV Inter-assay <15% CV
  • NGF: Intra-assay <10% CV Inter-assay <20%
  • PAI-1 (total): Intra-assay <10% CV Inter-assay <20% CV
  • Resistin: Intra-assay <10% CV Inter-assay <15% CV
  • TNFα: Intra-assay <10% CV Inter-assay <20% CV
Panel TypeMAGNETIC Metabolism
Applications
ApplicationThe Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.
Key Applications
  • Multiplexing
Application NotesFor optimal sensitivity, use an overnight (16-18 hour) incubation. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Adiponectin
  • HGF
  • IL-1β
  • IL-6
  • IL-8
  • Leptin
  • MCP-1
  • NGF
  • PAI-1 (Total)
  • Resistin
  • TNF-α
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • Adiponectin 97%
  • HGF 99%
  • IL-1β 99%
  • IL-6 100%
  • IL-8 99%
  • Leptin 90%
  • MCP-1 103%
  • NGF 101%
  • PAI-1 (total) 83%
  • Resistin 87%
  • TNFα 102%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • Adiponectin 15 pg/mL
  • HGF 3.0 pg/mL
  • IL-1β 2.0 pg/mL
  • IL-6 0.6 pg/mL
  • IL-8 0.2 pg/mL
  • Leptin 10 pg/mL
  • MCP-1 2.1 pg/mL
  • NGF 0.2 pg/mL
  • PAI-1 (total) 4.6 pg/mL
  • Resistin 4.4 pg/mL
  • TNFα 0.2 pg/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Adipnectin: 26 - 400,000 pg/mL
  • HGF: 6.4 – 100,000 pg/mL
  • IL-1β: 0.51 - 8,000 pg/mL
  • IL-6: 0.96 - 150,000 pg/mL
  • IL-8: 0.29 - 4,000 pg/mL
  • Leptin: 9.6 - 150,000 pg/mL
  • MCP-1: 0.96 - 150,000 pg/mL
  • NGF : 0.32 - 5,000 pg/mL
  • PAI-1: 9.6 - 150,000 pg/mL
  • Resistin: 6.4 - 100,000 pg/mL
  • TNFα: 0.38 - 6,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications

Documentation

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Título
PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

Protocols

Title
Human Adipocyte Magnetic Bead Panel

MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay Ficha datos de seguridad (MSDS)

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Ficha técnica de seguridad del material (MSDS) 

Referencias bibliográficas

Visión general referenciasPub Med ID
Plasma resistin levels are associated with homocysteine, endothelial activation, and nitrosative stress in obese youths.
Codoñer-Franch, P; Tavárez-Alonso, S; Porcar-Almela, M; Navarro-Solera, M; Arilla-Codoñer, Á; Alonso-Iglesias, E
Clinical biochemistry  47  44-8  2014

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96 well Template 2 Up
96 well Template Single
HADCYMAG-61K xPonent Protocol Template Version 3.1
HADCYMAG-61K xPonent Protocol Template Version Magpix
HADCYMAG-61K xPonent Standard Template Version 3.1
HADCYMAG-61K xPonent Standard Template Version Magpix
Quality Control Ranges: Human Adipocyte Magnetic Bead Panel -HADCYMAG-61K

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