MCVD2MAG-77K MILLIPLEX® MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

MCVD2MAG-77K
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Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
CXCL16, Endocan-1 (ESM-1), LIGHT, Follistatin, Oncostatin M (OSM), sCD40L, PIGF-2, Troponin I (TnI), Troponin T (TnT) M Mplex Luminex xMAP
Description
Catalogue NumberMCVD2MAG-77K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay
OverviewThe Milliplex® MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 contains all the components necessary to measure 9 biomarkers in any combination: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I and Troponin T. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background InformationCardiovascular disease (CVD), particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO). Now widely recognized, inflammatory mechanisms play a vital role in the initiation, maintenance and progression of vascular
disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. In addition, numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk factors.

The Milliplex® MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 contains all the components necessary to measure 9 biomarkers in any combination: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I and Troponin T. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
References
Product Information
Components
  • Mouse CVD Panel 2 Standard, 1 vial, lyophilized
  • Mouse CVD Panel 2 Quality Control 1, 1 vial, lyophilized
  • Mouse CVD Panel 2 Quality Control 2, 1 vial, lyophilized
  • Serum Matrix (0.08% Sodium Azide), 1 vial, lyophilized
  • Assay Buffer, 1 bottle, 30 mL
  • Bead Diluent, 1 bottle, 4 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Mouse CVD Panel 2 Detection Antibodies, 1 bottle, 5.5 mL
  • Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate
  • 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 20%
  • Intra-Assay: < 10%
Panel TypeMAGNETIC Cardiovascular
Applications
ApplicationThe MILLIPLEX® MAP Mouse Cardiovascular Disease Panel 2 is used for the simultaneous quantification of any of the following analytes in serum, plasma, or culture supernatant samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I, Troponin T.
Key Applications
  • Multiplexing
Application NotesThis assay is an overnight assay. A maximum of 10 μL per well of neat serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Mouse
Analytes Available
  • CXCL16
  • Endocan-1 (ESM-1)
  • LIGHT
  • Follistatin
  • Oncostatin M (OSM)
  • sCD40L
  • PIGF-2
  • Troponin I (TnI)
  • Troponin T (TnT)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • 84% - 106%
Physicochemical Information
Sensitivity
  • See kit protocol for individual analytes sensitivities.
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • PIGF-2: 4 – 3,000 pg/mL
  • Endocan-1: 14 – 10,000 pg/mL
  • CXCL16, Oncostatin M: 34 – 25,000 pg/mL
  • Troponin T: 69 – 50,000 pg/mL
  • CD40L: 137 – 100,000 pg/mL
  • Follistatin: 274 – 200,000 pg/mL
  • LIGHT, Troponin I: 686 – 500,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Mouse CVD Magnetic Bead Panel 2

MILLIPLEX® MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay Ficha datos de seguridad (MSDS)

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Licencias necesarias e Información técnica

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96 well Template 2 Up
96 well Template Single
Quality Control Ranges: Milliplex Mouse CVD Magnetic Bead Panel 2
White Paper: Further considerations of antibody validation and usage.

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