09-881 Anti-acetyl Cortactin Antibody

09-881
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-881
      DescriptionAnti-acetyl Cortactin Antibody
      Alternate Names
      • Amplaxin
      • Oncogene EMS1
      • Src substrate cortactin
      Background InformationCortactin is a cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

      Recent research efforts show cortactin is acetylated in vivo and is deacetylated by HDAC6 (Histone deacetylase 6). HDAC6 is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In recent studies it was demonstrated that HDAC6 binds cortactin and alters the ability of cortactin to bind F-actin by modulating a “charge patch” in its repeat region. Findings suggest that, in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin. The acetylation level of cortactin may correlate to tumor metastases and acetylated cortactin may be considered a potential metastasis marker.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-acetyl Cortactin Antibody is validated for use in Western Blotting, for the detection of acetyl Cortactin.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenConsensus sequence motif of acetylation sites of cortactin.
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes human acetylated Cortactin.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman and mouse. Predicted to react with rat and drosophila based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]
      Gene Symbol
      • CTTN
      • EMS1
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

      SUBUNIT STRUCTURE: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 By similarity. Interacts with PLXDC2.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Note: Associated with membrane ruffles and lamellipodia By similarity.

      SEQUENCE SIMILARITIES: Contains 7 cortactin repeats.

      Contains 1 SH3 domain.
      Molecular Weight~ 85 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

      Western Blot Analysis: 2.5 µg/ml of this antibody detected Cortactin on 10 µL of HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-acetyl Cortactin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl Cortactin Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl Cortactin2453216
      Anti-acetyl Cortactin - 20129662012966
      Anti-acetyl Cortactin - 20286042028604
      Anti-acetyl Cortactin - 21200962120096
      Anti-acetyl Cortactin - 22804782280478
      Anti-acetyl Cortactin - 24890402489040
      Anti-acetyl Cortactin - JH1784336JH1784336
      Anti-acetyl Cortactin - NG1722204NG1722204
      Anti-acetyl Cortactin - NG1845603NG1845603
      Anti-acetyl Cortactin - NG1865065NG1865065

      References

      Reference overviewPub Med ID
      Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading.
      Meiler, Eugenia, et al.
      PLoS ONE, 7: e33662 (2012)  2012

      Show Abstract
      22479425 22479425

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies