33-017 PI 3-Kinase HTRF™ Assay; 1920 wells

5 plates  Kit capacity: 1920 wells
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      Replacement Information
      Catalogue Number33-017
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPI 3-Kinase HTRF™ Assay; 1920 wells
      Product Information
      • 4X Reaction Buffer
      • PIP2 (Substrate; 1mM stock)
      • Stop Reagent A
      • Stop Reagent B
      • DM A Detection Reagent
      • DM B Detection Reagent
      • DM C Detection Reagent
      Detection methodHTRF
      ApplicationThe PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • MGC142163
      • PI3K
      • MGC142161
      • p110-alpha
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size5 plates
      Material PackageKit capacity: 1920 wells
      Transport Information
      Supplemental Information


      PI 3-Kinase HTRF™ Assay; 1920 wells SDS


      Safety Data Sheet (SDS) 

      PI 3-Kinase HTRF™ Assay; 1920 wells Certificates of Analysis

      TitleLot Number
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 24638882463888
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 18951181895118
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 20160592016059
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 20354912035491
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21198992119899
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21234642123464
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21234652123465
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21789522178952
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21789542178954
      PI3 Kinase 4-Step Assay Reagent 5-Plate Kit - 21789552178955


      Reference overviewPub Med ID
      Baculovirus production of fully-active phosphoinositide 3-kinase alpha as a p85alpha-p110alpha fusion for X-ray crystallographic analysis with ATP competitive enzyme inhibitors.
      Robert H Sinnamon,Patrick McDevitt,Beth L Pietrak,Vaughan R Leydon,Yu Xue,Ruth Lehr,Hongwei Qi,Matthew Burns,Patricia Elkins,Paris Ward,Giorgia Vincentini,Donald Fisher,Maggie Grimes,Martin Brandt,Kurt R Auger,Thau Ho,Kyung Johanson,Christopher S Jones,Benjamin Schwartz,Thomas D Sweitzer,Robert B Kirkpatrick
      Protein expression and purification  73  2010

      Show Abstract
      20457255 20457255


      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

      Data Sheet

      PI 3-Kinase HTRF Assay

      User Guides

      PI 3-Kinase (Class I) HTRF® Assay 5 plates (1920 wells)

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      33-016 PI 3-Kinase HTRF™ Assay; 385 wells Show Pricing & Availability
      33-036 PI 3-Kinase HTRF™ Assay; 10,000 wells Show Pricing & Availability
      33-037 PI 3-Kinase HTRF™ Assay; 50,000 wells Show Pricing & Availability

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