14-522M | Abl (T315I) Protein, active, 250 µg

14-522M
250 µg  Also available in 10 μg size (cat#14-522) and in bulk (cat# 14-522-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-522M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAbl (T315I) Protein, active, 250 µg
      OverviewN-terminal His6-tagged recombinant human Abl, residues 27-end containing the T315I mutation

      Contains the gatekeeper mutation T315I in the ATP-binding domain. This mutation decreases the sensitivity to Gleevec® by more than 200-fold as compared to the wild type sequence.
      References
      Product Information
      Applications
      ApplicationActive, N-terminal His6-tagged recombinant human Abl, residues 27-end containing the T315I mutation. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.
      Gene Symbol
      • ABL1
      • JTK7
      • ABL
      • p150
      • v-abl
      • c-ABL
      • bcr/abl
      Protein TargetAbl
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00519 # Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.
      COFACTOR: Magnesium or manganese.
      SIZE: 1130 amino acids; 122873 Da
      SUBUNIT: Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16 (By similarity). Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation. Isoform IB is myristoylated on Gly-2.
      DISEASE: SwissProt: P00519 # A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
      SIMILARITY: SwissProt: P00519 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight121kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Abltide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-522) and in bulk (cat# 14-522-K).
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-522 Abl (T315I) Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases