21-151 | Akt1/PKBα cDNA (activated) in pUSEamp

5 µg  
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      Replacement Information
      Catalogue Number21-151
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAkt1/PKBα cDNA (activated) in pUSEamp
      Product Information
      ApplicationTransfection grade eukaryotic expression vector containing Myc-His tagged mouse Akt1 (activated) under the control of the CMV promoter.
      Key Applications
      • Transfection
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Akt1 cDNA (activated) in pUSEamp (N-terminal myristoylation) - 2384687 2384687
      Akt1 cDNA (activated) in pUSEamp (N-terminal myristoylation) - 2272613 2272613
      Akt1 cDNA (activated) in pUSEamp - DAM1588138 DAM1588138
      Akt1 cDNA (activated) in pUSEamp - DAM1754383 DAM1754383
      Akt1 cDNA (activated) in pUSEamp -2500458 2500458
      Akt1/PKBalpha; cDNA (activated) in pUSEamp - 17869 17869
      Akt1/PKBalpha; cDNA (activated) in pUSEamp - 19300 19300
      Akt1/PKBalpha; cDNA (activated) in pUSEamp - 21797 21797
      Akt1/PKBalpha; cDNA (activated) in pUSEamp - 23972 23972


      Reference overviewApplicationPub Med ID
      Phosphorylation of huntingtin reduces the accumulation of its nuclear fragments.
      Simon C Warby,Crystal N Doty,Rona K Graham,Jonathan Shively,Roshni R Singaraja,Michael R Hayden
      Molecular and cellular neurosciences  40  2009

      Show Abstract
      18992820 18992820
      The E3 ligase TTC3 facilitates ubiquitination and degradation of phosphorylated Akt.
      Futoshi Suizu,Yosuke Hiramuki,Fumihiko Okumura,Mami Matsuda,Akiko J Okumura,Noriyuki Hirata,Masumi Narita,Takashi Kohno,Jun Yokota,Miyuki Bohgaki,Chikashi Obuse,Shigetsugu Hatakeyama,Toshiyuki Obata,Masayuki Noguchi
      Developmental cell  17  2009

      Show Abstract
      20059950 20059950
      Modulation of Akt kinase activity by binding to Hsp90
      Sato, S., et al
      Proc Natl Acad Sci U S A, 97:10832-7 (2000)  2000

      Activation Assay10995457 10995457
      Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation
      Kohn, A. D., et al
      J Biol Chem, 271:31372-8 (1996)  1996

      8940145 8940145