MABE90 | Anti-ASF1a Antibody, clone MPH7

MABE90
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE90
      DescriptionAnti-ASF1a Antibody, clone MPH7
      Alternate Names
      • Histone chaperone ASF1A
      • Anti-silencing function protein 1 homolog A
      • hAsf1
      • Asf1a
      • CCG1-interacting factor A
      • CIA
      • hCIA
      Background InformationASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement, removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication independent chromatin assembly.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-ASF1a Antibody, clone MPH7 (Mouse Monoclonal Antibody) validated in IP to detect ASF1a also known as Histone chaperone ASF1A, hAsf1 or Asf1a.
      Key Applications
      • Immunoprecipitation
      Application NotesThis antibody is not recommended for western blotting.
      Biological Information
      ImmunogenGST tagged recombinant protein corresponding to human ASF1a.
      CloneMPH7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes ASF1a, but not ASF1b (Ambagala, A.P., et al. (2009). J Virol. 83(1):200-209.)
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.
      Gene Symbol
      • ASF1A
      • CGI-98
      • HSPC146
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

      SUBUNIT STRUCTURE: Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1.

      SUBCELLULAR LOCATION: Nucleus.
      Molecular Weight~23 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in Jurkat cell lysate.

      Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated ASF1a from 100 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ASF1a, clone MPH7 - 2448419 2448419
      Anti-ASF1a, clone MPH7 - NRG1940785 NRG1940785
      Anti-ASF1a, clone MPH7 -2547227 2547227

      References

      Reference overviewPub Med ID
      Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.
      Ambagala, AP; Bosma, T; Ali, MA; Poustovoitov, M; Chen, JJ; Gershon, MD; Adams, PD; Cohen, JI
      Journal of virology  83  200-9  2009

      Show Abstract
      18971269 18971269

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies