AB1031 | Anti-Aggrecan Antibody

AB1031
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1031
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aggrecan Antibody
      Alternate Names
      • Cartilage-specific proteoglycan core protein
      • Cartilage-specific proteoglycan core protein
      • aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)
      • aggrecan proteoglycan
      • aggrecan proteoglycan
      • large aggregating proteoglycan
      Background InformationAggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Cartilage, neural tube, and brain tissue.
      PresentationRabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
      Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
      Applications
      ApplicationAnti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry(Paraffin):
      A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.

      Immunoprecipitation:
      A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
      Biological Information
      ImmunogenGST fusion protein containing amino acids 1177-1326 of mouse aggrecan.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB1031 is specific for mouse aggrecan; other species have not been tested.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Expected to cross react with Human and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Aggrecan 2475619
      Anti-Aggrecan - 2388819 2388819
      Anti-Aggrecan - 2458937 2458937
      Anti-Aggrecan - 1938408 1938408
      Anti-Aggrecan - 2000382 2000382
      Anti-Aggrecan - 2074020 2074020
      Anti-Aggrecan - 2198946 2198946
      Anti-Aggrecan - 2266459 2266459
      Anti-Aggrecan - 2293507 2293507
      Anti-Aggrecan - 2328304 2328304

      References

      Reference overviewApplicationSpeciesPub Med ID
      Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
      Suttkus, A; Rohn, S; Weigel, S; Glöckner, P; Arendt, T; Morawski, M
      Cell death & disease  5  e1119  2014

      Show Abstract
      Immunohistochemistry24625978 24625978
      Enriched housing enhances recovery of limb placement ability and reduces aggrecan-containing perineuronal nets in the rat somatosensory cortex after experimental stroke.
      Madinier, A; Quattromani, MJ; Sjölund, C; Ruscher, K; Wieloch, T
      PloS one  9  e93121  2014

      Show Abstract
      ImmunohistochemistryRat24664200 24664200
      Hyaluronan deficiency due to Has3 knock-out causes altered neuronal activity and seizures via reduction in brain extracellular space.
      Arranz, AM; Perkins, KL; Irie, F; Lewis, DP; Hrabe, J; Xiao, F; Itano, N; Kimata, K; Hrabetova, S; Yamaguchi, Y
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  6164-76  2014

      Show Abstract
      24790187 24790187
      Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays.
      Phillips, MD; Kuznetsov, SA; Cherman, N; Park, K; Chen, KG; McClendon, BN; Hamilton, RS; McKay, RD; Chenoweth, JG; Mallon, BS; Robey, PG
      Stem cells translational medicine  3  867-78  2014

      Show Abstract
      24855277 24855277
      Biglycan modulates angiogenesis and bone formation during fracture healing.
      Berendsen, AD; Pinnow, EL; Maeda, A; Brown, AC; McCartney-Francis, N; Kram, V; Owens, RT; Robey, PG; Holmbeck, K; de Castro, LF; Kilts, TM; Young, MF
      Matrix biology : journal of the International Society for Matrix Biology  35  223-31  2014

      Show Abstract
      Immunohistochemistry24373744 24373744
      Tenascin-R promotes assembly of the extracellular matrix of perineuronal nets via clustering of aggrecan.
      Morawski, M; Dityatev, A; Hartlage-Rübsamen, M; Blosa, M; Holzer, M; Flach, K; Pavlica, S; Dityateva, G; Grosche, J; Brückner, G; Schachner, M
      Philosophical transactions of the Royal Society of London. Series B, Biological sciences  369  20140046  2014

      Show Abstract
      25225104 25225104
      Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
      Mathieu, M; Iampietro, M; Chuchana, P; Guérit, D; Djouad, F; Noël, D; Jorgensen, C
      The Journal of biological chemistry  289  8402-12  2014

      Show Abstract
      24505142 24505142
      Mast cell-restricted, tetramer-forming tryptases induce aggrecanolysis in articular cartilage by activating matrix metalloproteinase-3 and -13 zymogens.
      Magarinos, NJ; Bryant, KJ; Fosang, AJ; Adachi, R; Stevens, RL; McNeil, HP
      Journal of immunology (Baltimore, Md. : 1950)  191  1404-12  2013

      Show Abstract
      Immunohistochemistry23797671 23797671
      Seizure-like activity in hyaluronidase-treated dissociated hippocampal cultures.
      Vedunova, M; Sakharnova, T; Mitroshina, E; Perminova, M; Pimashkin, A; Zakharov, Y; Dityatev, A; Mukhina, I
      Frontiers in cellular neuroscience  7  149  2013

      Show Abstract
      24062641 24062641
      Neurochemical mapping of the human hippocampus reveals perisynaptic matrix around functional synapses in Alzheimer's disease.
      Lendvai, Dávid, et al.
      Acta Neuropathol., 125: 215-29 (2013)  2013

      Show Abstract
      22961619 22961619

      Data Sheet

      Title
      Anti-Aggrecan - Data Sheet

      FAQ

      QuestionAnswer
      What is the best primary antibody dilution buffer? At Millipore ,we always recommend to dilute our primary antibody solutions in very simple buffers, usually only TBS or PBS without excess blocking proteins, detergents or other compounds, particularly preservatives such as azide (particularly important if using HRP detections systems: NEVER MIX AZIDE and HRP for the best results as azide is a very powerful inhibitor of HRP]. By keeping the primary antibody simple, this increase antibody-antigen interaction and can increase staining, and when sample has been properly blocked and prepared we have seen increase in background, and often see an improvement in the signal to noise ratio with simple, dilute primary antibody dilution buffers.

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MAB1581 Anti-Chondroitin Sulfate Proteoglycan Antibody, Core Protein Epitope, clone Cat-315 Show Pricing & Availability
      MAB19310 Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28 Show Pricing & Availability
      MAB2010 Anti-Cartilage Proteoglycan Antibody, fetal, clone HFPG-846 Show Pricing & Availability
      MAB2015 Anti-Cartilage Proteoglycan Antibody, adult, clone EFG-4 Show Pricing & Availability
      MAB2035 Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 Show Pricing & Availability
      MAB5284 Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 Show Pricing & Availability
      MABT110 Anti-Aggrecan (neoepitope) Antibody, clone BC-3 Show Pricing & Availability
      MABT153 Anti-Aggrecan Antibody (full length), clone 4D7.1 Show Pricing & Availability
      MABT83 Anti-Aggrecan Antibody, clone 6B4 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies