AB3559-50UL | Anti-Aquaporin 5 Antibody

AB3559-50UL
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3559-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aquaporin 5 Antibody
      Alternate Names
      • AQP5
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      ApplicationAnti-Aquaporin 5 Antibody detects level of Aquaporin 5 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:200 - 1:500 using ECL on rat lung membranes and mouse salivary gland.


      Immunohistochemistry on rat lung sections.


      Dilutions should be made using a carrier protein such as BSA (1-3%)


      Optimal working dilutions must be determined by the end user.


      SUGGESTED WESTERN BLOT PROTOCOL





      1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.


      2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.


      3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)


      4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).


      5. Destain with deionized water.


      6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for


      10 min, and filtered through glass filter (Gelman Acrodisc).


      7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.


      8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.


      9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.


      10. Wash 4 x 10 min. with PBS-0.1% tween 20.


      11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
      Biological Information
      ImmunogenPurified peptide from amino acids 251-265 of rat AQP5 (Accession number P47864).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Aquaporin 5 (AQP5).
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13.
      Gene Symbol
      • AQP5
      • AQP-5
      • Aquaporin-5
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55064 # Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
      SIZE: 265 amino acids; 28292 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: P55064 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
      SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-AQUAPORIN 5 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2395667 2395667
      RABBIT ANTI-AQUAPORIN 5 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2462746 2462746
      RABBIT ANTI-AQUAPORIN 5 -2661290 2661290
      RABBIT ANTI-AQUAPORIN 5 -2722840 2722840
      RABBIT ANTI-AQUAPORIN 5 -2780622 2780622
      RABBIT ANTI-AQUAPORIN 5 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2068429 2068429
      RABBIT ANTI-AQUAPORIN 5 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2326297 2326297
      RABBIT ANTI-AQUAPORIN 5, AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2837537

      Data Sheet

      Title
      RABBIT ANTI-AQUAPORIN 5 AFFINITY PURIFIED POLYCLONAL ANTIBODY