MABN1171 | Anti-Aquaporin-8, clone EPR8397, Rabbit Monoclonal Antibody

MABN1171
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1171
      DescriptionAnti-Aquaporin-8, clone EPR8397, Rabbit Monoclonal Antibody
      Alternate Names
      • Aquaporin-8
      • AQP-8
      • AQP8
      Background Information Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. A new water channel, Aquaporin-8, has been identified in rat pancreas and testis by homology cloning (Koyama et al., 1997). Aquaporin-8 (263 aa; 28 kDa) is also found in liver, colon and salivary glands. Aquaporin-8 (APQ8) is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      Applications
      ApplicationAnti-Aquaporin-8, clone EPR8397, Rabbit Monoclonal is an antibody against Aquaporin-8 for use in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A 1:100-500 dilution from a representative lot detected Aquaporin-8 in HeLa cells.

      Produced in collaboration with Epitomics™
      Biological Information
      ImmunogenSynthetic peptide corresponding to the extracellular domain of human Aquaporin-8.
      Epitopeextracellular domain
      CloneEPR8397
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityEquilibrium disassociation constant (KD) is 4.60 x 10 -11 M
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • AQP8
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, BxPC-3, and Caco-2 cell lysates.

      Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected Aquaporin-8 in HeLa, BxPC-3, and Caco-2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      AB3077 Anti-Aquaporin 8 Antibody, colon & pancreas only Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies