MAB4500-C | Anti-B23 Antibody, clone 3C9, Ascites Free

100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkWB, ICC, IHC, IPMPurifiedMonoclonal Antibody
      Catalogue NumberMAB4500-C
      DescriptionAnti-B23 Antibody, clone 3C9, Ascites Free
      Alternate Names
      • Nucleophosmin
      • B23
      • NPM
      • Nucleolar phosphoprotein B23
      • Nucleolar protein NO38
      • Numatrin
      Background InformationNucleophosmin (UniProt P06748; also known as B23, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin) is encoded by the NPM1 (also known as NPM) gene (Gene ID 4869) in human. Nucleophosmin (NPM) is a nucleolar protein that plays a role in ribosome biogenesis, nucleocytoplasmic transport, centrosome duplication, embryonic development, histone chaperone function, and transcriptional regulation. NPM is phosphorylated by Cdk2-cyclin E on Thr199 which causes the dissociation of NPM from the centrosome to allow duplication during the cell cycle. NPM is phosphorylated by the mitotic Cdk1-cyclin B complex on Thr234/237 during mitosis, resulting in its reassociation with the duplicated centrosomes. The human NPM1 gene transcribes three alternatrively spliced isoforms, isoforms 1&2 contain a nucleolar localization signal at their C-terminal end (W288/290 of isoform 1 and W259/261 of isoform 2), which is missing in the spliced isoform 3. In addition, a nuclear export signal is introduced, rendering the aberrant cytoplasmic location of this spliced isoform (NPMc).
      Product Information
      PresentationPurified mouse monoclonal IgMκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-B23 Antibody, clone 3C9, Ascites Free is an antibody against B23 for use in Western Blotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected B23 in 10 µg of HeLa and Jurkat cell lysates.
      Immunocytochemistry Analysis: Representative lots immunostained the nucleoli of paraformaldehyde-fixed, Triton X-100-permeabilized human cells by fluorescent immunocytochemistry (JIn, Y.Q., et al. (2014). Cell Cycle. 13(10):1627-1638; Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72; Bulycheva, T.I., et al. (2000). Tsitologiia. 42(10):944-954; Bulycheva, T.I., et al. (2000). Klin. Lab. Diagn. (1):37-38).
      Immunohistochemistry Analysis: A representative lot immunostained the nucleoli of dopaminergic (DA) neurons in formalin-fixed, paraffin-embedded human and mouse brain sections (Domanskyi, A., et al. (2011). FASEB J. 25(9):2898-2910; Rieker, C., et al. (2011). J. Neurosci. 12;31(2):453-460).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous B23/NPM and associated BRCA2 from COS-7 cell lysate (Wang, H.F., et al. (2011). Cancer Res. 71(1):68-77).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated B23 from HeLa cell lysates (Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72).
      Western Blotting Analysis: A representative lot detected the 35-40 kDa B23 doublet bands in HeLa and Ramos cell lysates as well as in HeLa B23 immunoprecipitate obtained with clone 3C9 (Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72).
      Biological Information
      ImmunogenDaudi nuclear extract.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 3C9 detected the 37/43 kDa nucleolar protein B23 located in the nucleolar granules in various cells, most intensely in actively proliferating cell cultures (Bulycheva, T.I., et al. (2000). Klin. Lab. Diagn. (1):37-38).
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NPM1
      • NPM
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~37 kDa observed.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected B23 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information