MABC199 | Anti-BRCA1 Antibody, clone MS110

MABC199
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC199
      DescriptionAnti-BRCA1 Antibody, clone MS110
      Alternate Names
      • Breast cancer type 1 susceptibility protein
      • RING finger protein 53
      Background InformationBreast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminus of human BRCA1.
      EpitopeN-terminus
      CloneMS110
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of BRCA1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BRCA1
      • RNF53
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa and ~85 kDa observed. Multiple isoforms exist at various molecular weights and may be subject to post-translational modification.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BRCA1, clone MS110 2484059
      Anti-BRCA1, clone MS110 - 2116707 2116707
      Anti-BRCA1, clone MS110 - 2300710 2300710
      Anti-BRCA1, clone MS110 - 2515709 2515709
      Anti-BRCA1, clone MS110 - 2537279 2537279
      Anti-BRCA1, clone MS110 - Q2015735 Q2015735
      Anti-BRCA1, clone MS110 -2505483 2505483
      Anti-BRCA1, clone MS110 -2551023 2551023
      Anti-BRCA1, clone MS110 -2649281 2649281
      Anti-BRCA1, clone MS110 -2718267 2718267

      References

      Reference overviewPub Med ID
      Evolutionary pathways in BRCA1-associated breast tumors.
      Martins, Filipe C, et al.
      Cancer Discov, 2: 503-11 (2012)  2012

      Show Abstract
      22628410 22628410
      Localization of human BRCA1 and its loss in high-grade, non-inherited breast carcinomas.
      Wilson, C A, et al.
      Nat. Genet., 21: 236-40 (1999)  1999

      Show Abstract
      9988281 9988281
      Location of BRCA1 in human breast and ovarian cancer cells.
      Scully, R, et al.
      Science, 272: 123-6 (1996)  1996

      8600523 8600523

      Brochure

      Title
      New Products: Volume 3, 2012

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      AP124P Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L Show Pricing & Availability
      07-434 Anti-BRCA1 Antibody Show Pricing & Availability
      07-635 Anti-phospho-BRCA1 (Ser1423) Antibody Show Pricing & Availability
      MAB4132 Anti-BRCA1 Antibody, clone BR64 Show Pricing & Availability
      MABC161 Anti-BRCA1 Antibody, clone MS13 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies