MAB3510P | Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated

MAB3510P
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AFC, ICC, IHCMHRPMonoclonal Antibody
      Description
      Catalogue NumberMAB3510P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BrdU Antibody, clone BU-1, Peroxidase conjugated
      Alternate Names
      • BrdU
      Background InformationBromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated.
      References
      Product Information
      FormatHRP
      PresentationLyophilized purified HRP conjugated IgG. Buffer= 10mM PBS containing 0.1% thimerosal and 15mg/ml BSA. Reconstititute with 100 microliters of sterile distilled water.Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
      Applications
      ApplicationAnti-BrdU Antibody, clone BU-1, Peroxidase conjugated is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC & IHC.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocyto/histochemistry: 1:300

      Dilution buffers should not contain sodium azide since it inhibits HRP activity.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBromodeoxyuridine
      Epitopeno acid or proteinase required
      CloneBU-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromodeoxyuridine does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine. MAB3510P does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU.
      IsotypeIgG2a
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months. Avoid freezing after reconsituition.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-BROMODEOXYURIDINE (BrdU) HRP CONJUGATED MONOCLONAL ANTIBODY - 2392251 2392251
      MOUSE ANTI-BROMODEOXYURIDINE (BrdU) -2669953 2669953
      MOUSE ANTI-BROMODEOXYURIDINE (BrdU) -2726744 2726744

      References

      Reference overviewPub Med ID
      S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment.
      Gonchoroff, N J, et al.
      J. Immunol. Methods, 93: 97-101 (1986)  1986

      Show Abstract
      3772110 3772110
      Immunofluorescent plasma cell labeling indices (LI) using a monoclonal antibody (BU-1).
      Greipp, P R, et al.
      Am. J. Hematol., 20: 289-92 (1985)  1985

      Show Abstract
      3904417 3904417

      Data Sheet

      Title
      Anti-Bromodeoxyuridine, No Acid or Proteinase Required, Clone BU-1, Peroxidase Conjugated - Data Sheet

      Related Products & Applications

      Alternative Format

      Catalogue Number Description  
      MAB3510 Anti-BrdU Antibody, clone BU-1 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies