MABF916 | Anti-CD8a (human) Antibody, AlexaFluor®488, clone HIT8a

100 tests  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHCMAlexaFluor®488Monoclonal Antibody
      Catalogue NumberMABF916
      DescriptionAnti-CD8a (human) Antibody, AlexaFluor®488, clone HIT8a
      Alternate Names
      • T-cell surface glycoprotein CD8 alpha chain
      • T-lymphocyte differentiation antigen T8/Leu-2
      • CD8a
      Background InformationCD8, a member of the immunoglobulin superfamily, is expressed on the majority of thymocytes, a subset of mature T lymphocytes, and NK cells (almost exclusively CD8a homodimers). CD8a acts as a co-receptor with MHC class I-restricted T cell receptors, and through its association with protein tyrosine kinase p56Ick, modulates antigen recognition and T cell activation as well as thymic development.

      Product Information
      PresentationPurified mouse conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.
      ApplicationDetect CD8 using this mouse monoclonal antibody, Anti-CD8a (human) Antibody, AlexaFluor®488, clone HIT8a validated for use in Flow Cytometry & IHC.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected human CD45RO in Immunohistochemistry. (Coppieters KT, et al. 2012.;Suzuki F, et al. 2012.)

      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CD8A
      • MAL
      Purification MethodAffinity
      UniProt Number
      Molecular Weight26 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe antibody was purified by affinity chromatography, and conjugated with AlexaFluor®488 under optimal conditions. The solution is free of unconjugated AlexaFluor®488 and unconjugated antibody. Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

      Flow Cytometry Analysis: 5 μL of this antibody detected CD8a in one million human peripheral blood lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD8a (human), AlexaFluor® 488, clone HIT8a - QVP1308305 QVP1308305


      Reference overviewPub Med ID
      Demonstration of islet-autoreactive CD8 T cells in insulitic lesions from recent onset and long-term type 1 diabetes patients.
      Coppieters, Ken T, et al.
      J. Exp. Med., 209: 51-60 (2012)  2012

      Show Abstract
      22213807 22213807
      Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
      Suzuki, Fumihito, et al.
      Arthritis Res. Ther., 14: R48 (2012)  2012

      Show Abstract
      22394569 22394569


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