ABT119 | Anti-CELSR1 Antibody

ABT119
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT119
      DescriptionAnti-CELSR1 Antibody
      Alternate Names
      • Cadherin EGF LAG seven-pass G-type receptor 1
      • Cadherin family member 9
      • Flamingo homolog 2
      • hFmi2
      Background InformationThe Flamingo/CELSR family of 7TM-cadherins are characterized with a symmetric protein distribution and polarized activity at neighboring epithelial cell interfaces along defined axes of planar cell polarity. As a component of the extracellular matrix, the CELSR1 protein has been found particularly at the basal surface of neuroepithelial cells within both the early neural tube and a less well-defined group of ventricular zone cells at the midline of the developing spinal cord. CELSR1 plays a role in orienting hair follicles along the anterior–posterior axis of the developing mouse epidermis, both during embryogenesis and postnatal development.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PC12 cell lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected CELSR1 in rat cerebellum, substancia nigra, and hippocampus tissue.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected CELSR1 in neurons of rat cortex tissue and in Purkinje cells and cells within the granular layer of rat cerebellum tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to human CELSR1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. This particular member is a developmentally regulated, neural-specific gene which plays an unspecified role in early embryogenesis.
      Gene Symbol
      • CELSR1
      • CDHF9
      • FMI2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor that may have an important role in cell/cell signaling during nervous system formation.

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

      POST-TRANSLATIONAL MODIFICATION: The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

      SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 9 cadherin domains. Contains 8 EGF-like domains. Contains 1 GPS domain. Contains 1 laminin EGF-like domain. Contains 2 laminin G-like domains.
      Molecular Weight~330 kDa observed. A second isoform at ~152 kDa may be observed in some lysates.
      An uncharacterized band at ~54 kDa may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC12 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected CELSR1 in 10 µg of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications