MAB3083-C | Anti-Calpain, small subunit Antibody, clone P-1 (Ascites Free)

MAB3083-C
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, R, CaWB, IH(P), ICC, IP, ACTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3083-C
      DescriptionAnti-Calpain, small subunit Antibody, clone P-1 (Ascites Free)
      Alternate Names
      • Calpain small subunit 1
      • CSS1
      • Calcium-activated neutral proteinase small subunit
      • CANP small subunit
      • Calcium-dependent protease small subunit
      • CDPS
      • Calcium-dependent protease small subunit 1
      • Calpain regulatory subunit
      • Calpain, small subunit
      Background InformationCalpain small subunit 1 (EC 3.4.22.17; UniProt P13135; also known as Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, Calpain 4, Calpain regulatory subunit, CANP small subunit, CDPS, CSS1) is encoded by the CAPNS1 (also known as CAPN4) gene (Gene ID 81664) in bovine species. Calpains are intracellular cysteine proteinases distributed in various tissues and cells. Functional calpains are composed of a large catalytic (80 kDa) and a small regulatory (30 kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca+2 concentrations and calpain II at millimolar Ca+2 concentrations. Calpain activity was initially characterized as “calcium-activated neutral protease” (CANP) detected in brain, lens of the eye, and other tissues. The name “calpain” was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
      Applications
      ApplicationAnti-Calpain, small subunit Antibody, clone P-1 (Ascites Free) is an antibody against Calpain, small subunit for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunoprecipitation and Activity Assay.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      • Activity Assay
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Calpain, small subunit in 10 µg of SHSY-5Y cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Calpain, small subunit in human kidney, human testis, and human prostate tissue.
      ELISA Analysis: 1:50,000 (against the immunogen).
      Western Blotting Analysis: A representative lot detected steady level of cellular calpain small subunit during the final stage of human CD34+ erythroid progenitor cells differentiation to reticulocytes (Patterson, T., et al. (2009). J. Biol. Chem. 284(21):14547-1457).
      Western Blotting Analysis: A representative lot detected upregulated calpain small subunit level in atrophied soleus muscle upon hind limbs immobilization by plaster cast among male rats (Sugiura, T., et al. (2003). J. Physiol. Sci. 56(6):393-399).
      Western Blotting Analysis: A representative lot detected calpain small subunit in human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830).
      Immunocytochemistry Analysis: A representative lot detected increrased plasma membrane calpain small subunit immunoreactivity following ionomcyin treatment of human large cell lung carcinoma LCLC-103H cells by fluorescent immunocytochemistry (Gil-Parrado, S., et al. (2003). J. Biol. Chem. 278(18):16336-16346).
      Immunoprecipitation Analysis: Representative lots immunoprecipitated calpain small subunit from human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830; Zhang, W., and Mellgren, R.L. (1996). Biochem. Biophys. Res. Commun. 227(3):891-896.).
      Activity Assay: A representative lot inhibited the proteolytic activity of bovine and canine calpain 2, as well as partially purified human erythrocyte calpain I as a result of increased Km and decreased Vmax toward the substrate casein (Mellgren, R.L., and Lane, R.D. (1988). Biochem. Biophys. Res. Commun. 954(2):154-160).
      Biological Information
      ImmunogenBovine myocardial m-calpain.
      CloneP1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with calpain small subunit.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Rat
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CAPNS1
      • CAPN4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~30 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Calpain, small subunit in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Calpain, small subunit, -Q2618556 Q2618556

      References

      Reference overviewPub Med ID
      Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cells.
      Patterson, ST; Li, J; Kang, JA; Wickrema, A; Williams, DB; Reithmeier, RA
      The Journal of biological chemistry  284  14547-57  2009

      Show Abstract Full Text Article
      19258325 19258325
      Estrogen administration attenuates immobilization-induced skeletal muscle atrophy in male rats.
      Sugiura, T; Ito, N; Goto, K; Naito, H; Yoshioka, T; Powers, SK
      The journal of physiological sciences : JPS  56  393-9  2006

      Show Abstract
      17052385 17052385
      Subcellular localization and in vivo subunit interactions of ubiquitous mu-calpain.
      Gil-Parrado, S; Popp, O; Knoch, TA; Zahler, S; Bestvater, F; Felgenträger, M; Holloschi, A; Fernández-Montalván, A; Auerswald, EA; Fritz, H; Fuentes-Prior, P; Machleidt, W; Spiess, E
      The Journal of biological chemistry  278  16336-46  2003

      Show Abstract
      12591934 12591934
      The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells.
      Zhang, W; Lane, RD; Mellgren, RL
      The Journal of biological chemistry  271  18825-30  1996

      Show Abstract
      8702541 8702541
      Calpain subunits remain associated during catalysis.
      Zhang, W; Mellgren, RL
      Biochemical and biophysical research communications  227  891-6  1996

      Show Abstract
      8886026 8886026
      Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit.
      Mellgren, RL; Lane, RD
      Biochimica et biophysica acta  954  154-60  1988

      Show Abstract
      2835105 2835105

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