MAB4709 | Anti-Caspase 9 Antibody, clone 2-23

MAB4709
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4709
      Replaces04-443
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 9 Antibody, clone 2-23
      Alternate Names
      • ICE-LAP6
      • Mch-6
      • Apaf-3
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.
      Applications
      ApplicationAnti-Caspase 9 Antibody, clone 2-23 is an antibody against Caspase 9 for use in IP, WB, IH(P).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1:1000 Immunohistochemistry (frozen and paraffin): 1:500 - 1:1000

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant human caspase-9 prodomain
      Clone2-23
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes 46-48 kDa full-length procaspase-9, human recombinant caspase-9 and procaspase-9 from human cell lysates. Highly specific to caspase-9 and shows no cross-reaction with other caspase family members. Activation of procaspase-9 by Apaf-1 in the cytochrome c pathway requires proteolytic cleavage to generate active caspase-9. Once activated, capsase-9 initiates a caspase cascade involving the downstream executioner caspases 3, 6 and 7.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
      Gene Symbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • MCH6
      • APAF3
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC 3.4.22.62 [Contains: Caspase-9 subunit p35
      • Caspase-9 subunit p10].
      Non-Reactive Species
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
      SIZE: 416 amino acids; 46281 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
      TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
      PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CASPASE-9 - 2836169 2836169
      MOUSE ANTI-CASPASE-9 -2774115 2774115
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 2135105 2135105
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 2140019 2140019
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 1993763 1993763
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 2181266 2181266
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 2246766 2246766
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY - 2352370 2352370
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY -2633743 2633743
      MOUSE ANTI-CASPASE-9 MONOCLONAL ANTIBODY -2653094 2653094

      References

      Reference overviewPub Med ID
      Uncoupling oxidative/energy metabolism with low sub chronic doses of 3-nitropropionic acid or iodoacetate in vivo produces striatal cell damage.
      Rodríguez, E; Rivera, I; Astorga, S; Mendoza, E; García, F; Hernández-Echeagaray, E
      International journal of biological sciences  6  199-212  2010

      Show Abstract
      20440403 20440403

      Data Sheet

      Title
      Anti-Caspase 9, clone 2-23 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies