06-1292 | Anti-Cep63 Antibody

06-1292
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1292
      DescriptionAnti-Cep63 Antibody
      Alternate Names
      • Centrosomal protein of 63 kDa
      Background InformationCentrosomal protein 63 (Cep63) serves an ATM and ATR substrate for spindle assembly activation following chromosomal breakage. It is shown that inactivation of the CEP63 gene in avian DT40 cells impairs spindle assembly and prevents ATM- and ATR-dependent effects on mitosis. Cep63 is also reported to bind to and recruit Cdk1 to centrosomes and is thought to be involved the regulation mitotoic entry, centrosome amplification, and genome maintentance.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK 293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Cep63 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Cep63 also known as Centrosomal protein of 63 kDa.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected Cep63 in U20S cells. (Nicola Brown, CRUK Clare Hall Laboratories, London, UK)
      Biological Information
      ImmunogenRecombinant protein corresponding to human Cep63.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein with six coiled-coil domains. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. Several alternatively spliced transcript variants have been found, but their biological validity has not been determined.
      Gene Symbol
      • CEP63
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Required for normal spindle assembly. Plays a role in DNA damage response: following DNA double strand breaks (DSBs), it is delocalized from centrosomes, leading to inactivate spindle assembly and delay mitotic progression By similarity.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton > centrosome.

      SEQUENCE SIMILARITIES: Belongs to the CEP63 family.
      Molecular Weight~63 kDa observed. Different isoforms or uncharacterized bands may be observed in some endogenous cell lysates. Experiments using transfected cells and siRNA have confirmed detection of Cep63.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: A 1: 500 dilution of this antibody detected Cep63 in 10 µg of HEK 293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cep63 - 2123674 2123674
      Anti-Cep63 - 2166827 2166827
      Anti-Cep63 - 2460449 2460449
      Anti-Cep63 - 06-1292 06-1292
      Anti-Cep63 - 2030401 2030401
      Anti-Cep63 - 2202473 2202473
      Anti-Cep63 - 2273982 2273982
      Anti-Cep63 - 2325143 2325143
      Anti-Cep63 - NRG1916845 NRG1916845
      Anti-Cep63 -2619406 2619406

      References

      Reference overviewPub Med ID
      Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.
      Shukla, A; Kong, D; Sharma, M; Magidson, V; Loncarek, J
      Nature communications  6  8077  2015

      Show Abstract
      26293378 26293378

      Brochure

      Title
      New Products: Volume 3, 2012