06-1289 | Anti-Cyclin T1 Antibody

06-1289
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBShSerumPolyclonal Antibody
      Description
      Catalogue Number06-1289
      DescriptionAnti-Cyclin T1 Antibody
      Alternate Names
      • CDK9-associated C-type protein
      • Human immunodeficiency virus-1 expression
      • cyclin C-related protein
      • cyclin T1
      • cyclin T1b
      • human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
      • subunit of positive elongation transcription factor b
      Background InformationCyclin T1, which is also known as CCNT, is a member of the cyclin family and functions as a CDK kinase regulator. Cyclin T1 is one of regulatory cyclins (T1, T2a, T2b, and K) that is closely associated with CDK9 kinase forming a heterodimer called P-TEFb. This kinase complex is involved in phosporylating the carboxyl-terminal domain (CTD) of the large RNA polymerase II subunit which stimulate the elongation upon initiation. The cyclin T1/CDK9 complex has also been referred to as tat-associated kinase. Tat is an HIV protein that targets the cyclin T1/CDK9 complex to activate the transcription of the virus.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationSheep polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1000 dilution from a previous lot detected Cyclin T1 in 10 µg of HEK293, HeLa, HepG2, Jurkat, and PC3 cell lysates.
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human Cyclin T1.
      EpitopeUnknown
      HostSheep
      SpecificityThis antibody recognizes Cyclin T1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse.
      Rat (85% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. [provided by RefSeq]
      Gene Symbol
      • CCNT1
      • CCNT
      • CYCT1
      • CycT1
      • Cyclin-T
      • HIVE1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

      SUBUNIT STRUCTURE: Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Ubiquitously expressed.

      MISCELLANEOUS: Interaction between Tat and cyclin-T1 requires zinc.

      SEQUENCE SIMILARITIES: Belongs to the cyclin family. Cyclin C subfamily.
      Molecular WeightCyclin T1 is expected to be ~85 kDa. This antibody detects a ~85 kDa band in most cell lines. It also detects the degraded forms of Cyclin T1 (~70 kDa, ~60 kDa, et al), which is beneficial for IP applications. According to independent laboratory, this antibody can immunoprecipitate multiple forms of Cyclin T1.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: A 1:1000 dilution of this antibody detected Cyclin T1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin T1 - NG1895577 NG1895577
      Anti-Cyclin T1 - NRG1644431 NRG1644431
      Anti-Cyclin T1 - NRG1644433 NRG1644433
      Anti-Cyclin T1 -2688506 2688506

      References

      Reference overviewApplicationPub Med ID
      The mechanism of release of P-TEFb and HEXIM1 from the 7SK snRNP by viral and cellular activators includes a conformational change in 7SK.
      Krueger, BJ; Varzavand, K; Cooper, JJ; Price, DH
      PloS one  5  e12335  2010

      Show Abstract
      Western Blotting20808803 20808803