ABE620 | Anti-EIF4A2 Antibody

ABE620
150 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE620
      DescriptionAnti-EIF4A2 Antibody
      Alternate Names
      • Eukaryotic initiation factor 4A-II
      • eIF-4A-II
      • eIF4A-II
      • ATP-dependent RNA helicase eIF4A-2
      • EIF4A2
      Background InformationEukaryotic initiation factor 4A-II, (EIF4A2), or ATP-dependent RNA helicase eIF4A-2 is the critical RNA helicase protein required for mRNA cap recognition and mRNA binding to the ribosome. EIF4A2 is part of a multi-subunit complex that allows translation of mRNAs to begin. EIF4A2 unwinds the RNA secondary structure in the 5’-UTR region and thus allows the small ribosomal subunit to bind the mRNA and begin scanning for the initiator codon. EIF4A2 also plays a key role in mRNA expression inhibition. Recent research demonstrates that inhibition of EIF4A2 function via microRNA inhibition is required first, which then leads to mRNA destabilization and ultimately translation suppression. EIF4A2 is expressed in all tissues, as well as in cancerous tumors. Expression is very high in ovary, followed by liver, kidney, brain and pancreas in lower amounts. EIF4A2 is often used as a “housekeeping” marker as well because of its wide expression.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.
      Applications
      ApplicationAnti-EIF4A2 Antibody is a highly specific rabbit polyclonal antibody, that targets EIF4A2 & has been tested in western blotting & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:25 dilution from a representative lot detected EIF4A2 in HepG2 cells.
      Biological Information
      ImmunogenGST-tagged fusion protein corresponding to human EIF4A2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • EIF4A2
      • DDX2B
      • EIF4F
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~46 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected EIF4A2 in MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size150 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies