AB7144F | Anti-Fibrinogen Antibody, FITC conjugated

AB7144F
2 mL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, ID, WBShFITCPolyclonal Antibody
      Description
      Catalogue NumberAB7144F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fibrinogen Antibody, FITC conjugated
      References
      Product Information
      FormatFITC
      PresentationThe antibody is supplied in 20mM Tris, 70mM NaCl, pH 8.0, with 0.1% sodium azide as preservative.
      Applications
      ApplicationDetect Fibrinogend using this Anti-Fibrinogen Antibody, FITC conjugated validated for use in IF, ID & WB.
      Key Applications
      • Immunofluorescence
      • Immunodiffusion
      • Western Blotting
      Application NotesWORKING DILUTION

      The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:

      1. Incident light excitation

      Direct immunofluorescence: 1/10 - 1/80

      Indirect immunofluorescence: 1/20 - 1/400

      2. Transmitted light excitation

      Direct immunofluorescence: 1/5 - 1/40

      Indirect immunofluorescence: 1/10 - 1/80
      Biological Information
      ImmunogenPurified fibrinogen derived from pooled human plasma.
      HostSheep
      SpecificityPrior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.

      LABELLING RATIO

      Fluorescein:antibody molar ratio: 2.8 - 3.3

      Absorbance ratio (A493/A276): 0.78 + 0.05
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus.
      Gene Symbol
      • FGA
      • MGC119423
      • MGC119425
      • Fib2
      • MGC119422
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02671 # Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
      SIZE: 866 amino acids; 94973 Da
      SUBUNIT: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.
      SUBCELLULAR LOCATION: Secreted.
      DOMAIN: SwissProt: P02671 A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
      PTM: The alpha chain is not glycosylated. & The alpha chain binds by 2-4 cross-links to the amino end of fibronectin. & About one-third of the alpha chains in the molecules in blood were found to be phosphorylated. & Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon- (gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      DISEASE: SwissProt: P02671 # Defects in FGA are a cause of congenital afibrinogenemia [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. & Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias. & Defects in FGA are a cause of hereditary renal amyloidosis [MIM:105200].
      SIMILARITY: Contains 1 fibrinogen C-terminal domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +2 to +8°C, for up to 6 months, undiluted. Protect from light.

      WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size2 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-HUMAN FIBRINOGEN 2462812
      SHEEP ANTI-HUMAN FIBRINOGEN IgG FRACTION FLUORESCEIN CONJUGATED - 2047671 2047671
      SHEEP ANTI-HUMAN FIBRINOGEN IgG FRACTION FLUORESCEIN CONJUGATED - 2437370 2437370
      SHEEP ANTI-HUMAN FIBRINOGEN -2514987 2514987
      SHEEP ANTI-HUMAN FIBRINOGEN -2643574 2643574
      SHEEP ANTI-HUMAN FIBRINOGEN -2685233 2685233
      SHEEP ANTI-HUMAN FIBRINOGEN -2794894 2794894
      SHEEP ANTI-HUMAN FIBRINOGEN IgG FRACTION FLUORESCEIN CONJUGATED 2897162
      SHEEP ANTI-HUMAN FIBRINOGEN IgG FRACTION FLUORESCEIN CONJUGATED - 2012366 2012366
      SHEEP ANTI-HUMAN FIBRINOGEN IgG FRACTION FLUORESCEIN CONJUGATED - 2018064 2018064

      References

      Reference overviewPub Med ID
      Sensitive immunoassays of nitrated fibrinogen in human biofluids.
      Zhiwen Tang,Hong Wu,Dan Du,Jun Wang,Hua Wang,Wei-jun Qian,Diana J Bigelow,Joel G Pounds,Richard D Smith,Yuehe Lin
      Talanta  81  2010

      Show Abstract
      20441955 20441955

      Data Sheet

      Title
      Anti-Fibrinogen, FITC Conjugated - Data Sheet