MAB1794 | Anti-Human B cells (CD19) Antibody, clone FMC63

MAB1794
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1794
      ReplacesCBL141
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Human B cells (CD19) Antibody, clone FMC63
      OverviewBIOCHEMISTRY:

      p I = 7.0
      Alternate Names
      • B-lymphocyte Surface Antigen B4
      References
      Product Information
      FormatPurified
      PresentationPurified from mouse ascitic fluid. The antibody is supplied in 25 mM Tris-HCl buffer, 0.4 M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
      Applications
      ApplicationThis Anti-Human B cells CD19 Antibody, clone FMC63 is validated for use in FC, IF for the detection of CD19.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      Application NotesThis antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.

      SUGGESTED USAGE DILUTION

      Flow cytometry and Indirect Immunofluorescence 1:25

      Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
      Biological Information
      ImmunogenRaji cells
      CloneFMC63
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.

      Cell reactivity:

      Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.

      Clinical:

      B cell chronic lymphocytic leukemia (B-CLL) +

      Pro lymphocytic leukemia (PLL) +

      Hairy cell leukemia (HCL) +

      Common acute lymphoblastic leukemia (CALL) +

      Pre-B acute lymphoblastic leukemia (pre-B-ALL) +

      NULL-acute lymphoblastic leukemia (NULL-ALL) +

      T common lymphocytic leukemia (T-CLL) -
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
      Gene Symbol
      • CD19
      • B4
      • MGC12802
      • Leu-12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
      SIZE: 556 amino acids; 61128 Da
      SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].
      SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

      WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Mouse (IgG2a) monoclonal antibody : Human B cells (CD19), purified - ME9CN4562 ME9CN4562
      Mouse (IgG2a) monoclonal antibody : Human B cells (CD19), purified - ME9EN5636 ME9EN5636
      Mouse monoclonal antibody: CD19, purified -2677232 2677232
      Mouse monoclonal antibody: CD19, purified -2787708 2787708

      References

      Reference overviewPub Med ID
      Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools.
      Díez, JM; Bauman, E; Gajardo, R; Jorquera, JI
      Stem cell research & therapy  6  28  2015

      Show Abstract
      25889980 25889980

      Data Sheet

      Title
      Anti-CD19, clone FMC63 - Data Sheet

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      Alternative Format

      Catalogue Number Description  
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies