ABF117 | Anti-IFIT1/p56 Antibody

ABF117
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICC, FCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABF117
      DescriptionAnti-IFIT1/p56 Antibody
      Alternate Names
      • Interferon-induced protein with tetratricopeptide repeats 1
      • IFIT-1
      • Glucocorticoid-attenuated response gene 16 protein
      • GARG-16
      • Interferon-induced 56 kDa protein
      • IFI-56K
      • P56
      Background InformationIFIT1, also known as GARG-16, Glucocorticoid-attenuated response gene 16 protein or Interferon-induced 56 kDa protein, IFI-56K, or P56, and encoded by the gene lfit1/Garg16, Ifi56, Isg56, is an interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. IFIT1 directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). IFIT1 is a component of an interferon-dependent multiprotein complex that is at least composed of IFIT1, IFIT2 and IFIT3 and interacts with EIF3F, RPL15, TMEM173 and EEF1A1 and is important in antiviral defense, and innate immune response. IFIT1 is localized to the cytoplasm and expressed by most cells, particularly in the immune system and epithelial cells.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationDetect IFIT1 using this rabbit polyclonal antibody, Anti-IFIT1/p56 Antibody validated for use in western blotting, ICC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected IFIT1/p56 in 10 µg of TF-1 and MOLT-4 cell lysates.

      Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in HT1080 cell lysates transfected with mouse p56 vector and not in HT1080 cell lysates transfected with empty vector (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated wild type Stat1 MEF cell lysates and not in IFN-beta treated Stat1 knockdown MEF cell lsyates (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated wild type p56 MEF cell lysates and not in IFN-Beta treated p56 knockdown MEF cell lysates (Fensterl, V., et al. (2012). PLoS Pathog. 8(5):e1002712.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated MEF cell lysates (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.).

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in bone marrow cells and splenocyotes upon injection with dsRNA, IFN-alpha, and VSV (Terenzi, F., et al. (2007). J Virol. 81(16):8656-8665.).

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected IFIT-1/p56 in IFN-Beta treated CD3+ mature T cells, and in IFN-Beta treated myeloid Dendritic cells, but not in IFN-Beta treated plasmacytoid Dendritic cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053.).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse IFIT1/p56.
      HostRabbit
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ifit1
      • Garg16
      • Ifi56
      • Isg56
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse ovary tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IFIT1/p56 in 10 µg of mouse ovary tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IFIT1/p56 - Q2315813 Q2315813

      References

      Reference overviewPub Med ID
      Interferon-induced Ifit2/ISG54 protects mice from lethal VSV neuropathogenesis.
      Fensterl, V; Wetzel, JL; Ramachandran, S; Ogino, T; Stohlman, SA; Bergmann, CC; Diamond, MS; Virgin, HW; Sen, GC
      PLoS pathogens  8  e1002712  2012

      Show Abstract
      22615570 22615570
      Novel characteristics of the function and induction of murine p56 family proteins.
      Fensterl, V; White, CL; Yamashita, M; Sen, GC
      Journal of virology  82  11045-53  2008

      Show Abstract
      18768971 18768971
      Tissue-specific and inducer-specific differential induction of ISG56 and ISG54 in mice.
      Terenzi, Fulvia, et al.
      J. Virol., 81: 8656-65 (2007)  2007

      Show Abstract
      17553874 17553874

      Brochure

      Title
      New Products Flyer- Feb 2014 - Vol 1