06-1066 | Anti-IRF-3 Antibody

06-1066
100 µL  
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      Overview

      Replacement Information
      Replacement InformationMABF21 is the recommended replacement for Discontinued catalog number 06-1066

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1066
      DescriptionAnti-IRF-3 Antibody
      Alternate Names
      • interferon regulatory factor 3
      Background InformationInterferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.
      References
      Product Information
      FormatUnpurified
      Control
      • K562 cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-IRF-3 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect IRF-3 also known as interferon regulatory factor 3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenLinear peptide corresponding to human IRF-3.
      EpitopeUnknown
      HostRabbit
      SpecificityThis antibody recognizes IRF-3.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
      Gene Symbol
      • IRF-3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
      SUBUNIT STRUCTURE: Homodimer; phosphorylation-induced. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
      TISSUE SPECIFICITY: Expressed constitutively in a variety of tissues.
      PTM: Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
      SEQUENCE SIMILARITIES: Belongs to the IRF family.
      Contains 1 tryptophan pentad repeat DNA-binding domain.
      Molecular Weight~47 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.
      Western Blot Analysis: A 1:1,000 of this antibody detected IRF-3 on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies