MAB2513 | Anti-Integrin α1β1 Antibody, clone RA1.1

MAB2513
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2513
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α1β1 Antibody, clone RA1.1
      Alternate Names
      • VLA-1
      References
      Product Information
      FormatPurified
      PresentationLiquid at 1 mg/mL in 0.02M PB + 0.25M NaCl + 0.1% NaN3 , pH 7.6.
      Applications
      ApplicationAnti-Integrin α1β1 Antibody, clone RA1.1 detects level of Integrin α1β1 & has been published & validated for use in FC, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      Applications Not Recommended
      • Western Blotting
      Application NotesImmunoprecipitation of VLA-1 integrin from detergent cell lysates. Suggested dilution: 2 μg per 10E6 cell equivalent.

      Flow cytometry: 10 μg/mL

      Immunohistochemistry: Detects VLA-1 in acetone-fixed frozen tissue sections from gut, heart, and lungs. Suggestion dilution: 20 μg/mL.

      Function: MAB2513 does not inhibit VLA-1 mediated adhesion to matrix proteins.

      Not effective for Western blot.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe antibody is secreted by a hybridoma prepared by fusion of spleen cells from Balb/c mice, immunized with rat pheochromocytoma PC12 cells with myeloma P3X63ag9.653 cells.
      CloneRA1.1
      HostMouse
      SpecificityAntibody MAB2513 is directed against rat integrin a1b1 (VLA-1), a receptor for collagen and laminin. Antibody specificity has been confirmed by 1) its ability to immunoprecipitate beta1 (Mr~200 kDa) and b1 (Mr~120 kDa) from (125)I-surface labeled PC12 cells and 2) reciprocal depletion of VLA-1 antigen from cell lysate with antiserum against the cytoplasmic domain of a1 integrin subunit. Analysis of immunoprecipitation by SDS-PAGE was carried out under non-reducing conditions using a 6% gel.

      The antibody will not recognize the individual integrin subunits, only the VLA-1 complex. The antibody is not effective in western blots of VLA-1 for this reason, only immunoprecipitation or staining (acetone fixed only).
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
      Gene Symbol
      • ITGA1
      • CD49a
      • VLA1
      • VLA-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen.
      SIZE: 1179 amino acids; 130848 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for short term storage only. Maintain frozen in aliquots at -20°C for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT INTEGRIN a1B1 [VLA-1] MONOCLONAL ANTIBODY 2926495
      MOUSE ANTI-RAT INTEGRIN α1 ß1 [VLA-1] - 2513552 2513552
      MOUSE ANTI-RAT INTEGRIN α1β1 [VLA-1] -2694558 2694558
      MOUSE ANTI-RAT INTEGRIN α1β1 [VLA-1] -2771404 2771404
      MOUSE ANTI-RAT INTEGRIN α1β1 [VLA-1] -2776747 2776747
      MOUSE ANTI-RAT INTEGRIN 11 [VLA-1] -2547189 2547189

      References

      Reference overviewPub Med ID
      Developmental and injury-induced expression of alpha1beta1 and alpha6beta1 integrins in the rat spinal cord.
      Baker, KA; Hagg, T
      Brain research  1130  54-66  2007

      Show Abstract
      17161391 17161391
      Involvement of alpha1beta1 integrin in insulin-like growth factor-1-mediated protection of PC12 neuronal processes from tumor necrosis factor-alpha-induced injury.
      Jin Ying Wang, Maja Grabacka, Cezary Marcinkiewicz, Izabella Staniszewska, Francesca Peruzzi, Kamel Khalili, Shohreh Amini, Krzysztof Reiss
      Journal of neuroscience research  83  7-18  2006

      Show Abstract
      16307448 16307448

      Data Sheet

      Title
      Anti-Integrin alpha1beta1, clone RA1.1 - Data Sheet

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-246 Anti-Integrin α1 Antibody, clone 5E8D9 Show Pricing & Availability
      AB1934 Anti-Integrin α1 Subunit Antibody, cytoplasmic/intracellular Show Pricing & Availability
      MAB1973 Anti-Integrin α1 Antibody, clone FB12 Show Pricing & Availability
      MAB1973Z Anti-Integrin α1 Antibody, clone FB12, azide free Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies